CORE--Molecular Biology/Gene Expression Core

分子生物学/基因表达核心为DHRC研究人员提供了一套互补的最先进的服务和技术，以及一个用于在多个水平上分析胃肠道细胞和组织中基因表达模式的集成框架。DNA微阵列、激光捕获显微切割和实时聚合酶链式反应等新技术彻底改变了我们研究特定细胞和组织亚群中数千个基因表达的能力。一个强大的生物信息学和统计基础设施对于分析和管理如此丰富的实验数据集来源是必要的。然而，对于个人调查人员来说，获得其中许多较新的新兴技术、仪器和分析工具的机会有限，而且成本高昂。通过提供具有成本效益的访问和专业知识 这些作为核心的新兴技术将加强个别研究人员的研究努力，并促进民主人权委员会成员之间的新合作。分子生物学/基因表达核心的具体目标是：1)通过提供咨询、培训和获得一套互补的服务，以具有成本效益的、高通量的方式分析基因表达模式，从而增强DHRC成员的研究能力；2)提供一个易于使用的核心设施，其中统一的质量控制可被系统地应用于高通量测定生物组织和细胞中的基因表达水平；3)开发和验证用于量化细胞和组织中基因表达的新的分析方法；4)为DHRC调查人员提供生物信息学和统计学解释方面的培训和专业知识 基因芯片和其他基因表达数据集；5)通过关于胃肠道细胞和组织基因表达分析原理和方法的讲座和正式咨询，促进将新兴新技术纳入DHRC调查研究计划；6)根据DHRC成员的需求，持续评估分子生物学/基因表达核心提供的服务的质量、成本效益和效用，以便分配可用的资源，为DHRC成员带来最大利益。

The Molecular Biology/Gene Expression Core provides DHRC investigators with a complementary set of state-of-the-art services and technologies and an integrated framework for analysis of patterns of gene expression in gastrointestinal cells and tissues at multiple levels. New technologies such as DNA microarray, laser capture microdissection, and real-time PCR have revolutionized our ability to study the expression of thousands of genes in defined subsets of cells and tissues. A robust bioinformatics and statistical infrastructure is necessary to analyze and manage such a rich source of experimental data sets. However, access to many of these newer emerging technologies, instrumentation, and analytical tools has been limited and expensive for individual investigators. By providing cost-effective access and expertise in these emerging technologies the core will enhance individual investigator's research efforts as well as foster new collaborations among DHRC members. The specific aims of the Molecular Biology/Gene expression core are to: 1) Enhance the research capabilities of DHRC members by providing consultation, training, and access to a complementary set of services for analyzing patterns of gene expression in a cost-effective, high-throughput manner; 2) Provide an easily accessible core facility where uniform quality controls can be systematically applied to high throughput determination of levels of gene expression in biological tissues and cells; 3) Develop and validate new assays for quantifying expression of genes in cells and tissues; 4) Provide DHRC investigators with training and expertise in bioinformatics and statistical interpretation of microarray and other gene expression data sets; 5) Facilitate integration of emerging new technologies into DHRC investigators research programs, through lectures and formal consultations on principles and methods of analysis of gene expression in gastrointestinal cells and tissues; 6) Perform ongoing evaluation of the quality, cost-effectiveness, and utility of services provided by the Molecular Biology/Gene Expression Core in relation to the needs of the DHRC membership so that the available resources can be allocated to bring the most benefit to DHRC members.