CORE--Molecular Biology/Gene Expression Core

分子生物学/基因表达核心为DHRC研究人员提供了一套补充的最先进的服务和技术，以及一个综合框架，用于分析胃肠道细胞和组织中多个水平的基因表达模式。DNA微阵列、激光捕获显微解剖和实时PCR等新技术彻底改变了我们研究细胞和组织中特定亚群中数千种基因表达的能力。一个强大的生物信息学和统计基础设施是分析和管理如此丰富的实验数据集的必要条件。然而，这些新兴的技术、仪器和分析工具对个体研究者来说是有限和昂贵的。通过提供具有成本效益的途径和专业知识

The Molecular Biology/Gene Expression Core provides DHRC investigators with a complementary set of state-of-the-art services and technologies and an integrated framework for analysis of patterns of gene expression in gastrointestinal cells and tissues at multiple levels. New technologies such as DNA microarray, laser capture microdissection, and real-time PCR have revolutionized our ability to study the expression of thousands of genes in defined subsets of cells and tissues. A robust bioinformatics and statistical infrastructure is necessary to analyze and manage such a rich source of experimental data sets. However, access to many of these newer emerging technologies, instrumentation, and analytical tools has been limited and expensive for individual investigators. By providing cost-effective access and expertise in these emerging technologies the core will enhance individual investigator's research efforts as well as foster new collaborations among DHRC members. The specific aims of the Molecular Biology/Gene expression core are to: 1) Enhance the research capabilities of DHRC members by providing consultation, training, and access to a complementary set of services for analyzing patterns of gene expression in a cost-effective, high-throughput manner; 2) Provide an easily accessible core facility where uniform quality controls can be systematically applied to high throughput determination of levels of gene expression in biological tissues and cells; 3) Develop and validate new assays for quantifying expression of genes in cells and tissues; 4) Provide DHRC investigators with training and expertise in bioinformatics and statistical interpretation of microarray and other gene expression data sets; 5) Facilitate integration of emerging new technologies into DHRC investigators research programs, through lectures and formal consultations on principles and methods of analysis of gene expression in gastrointestinal cells and tissues; 6) Perform ongoing evaluation of the quality, cost-effectiveness, and utility of services provided by the Molecular Biology/Gene Expression Core in relation to the needs of the DHRC membership so that the available resources can be allocated to bring the most benefit to DHRC members.