CORE--Molecular Biology/Gene Expression Core

CORE--分子生物学/基因表达核心

• 批准号: 7447857
• 负责人: STEVEN M COHN
• 金额: $ 18.06万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7447857
• 关键词: Area; Arts; Bioinformatics; Biological; Biological Assay; Cells; Collaborations; Consultations; Core Facility; DNA Microarray Chip; DNA Microarray format; Data Set; Data Sources; Emerging Technologies; Evaluation; Fostering; Gene Expression; Gene Expression Profiling; Genes; Goals; Health; Individual; Inflammation; Malignant Neoplasms; Methods; Mission; Molecular Biology; Pattern; Polymerase Chain Reaction; Quality Control; Research; Research Infrastructure; Research Personnel; Resources; Services; Source; Targeted Research; Technology; Time; Tissues; Training; analytical tool; cost; cost effectiveness; gastrointestinal; instrumentation; laser capture microdissection; lectures; member; new technology; programs; research study

The Molecular Biology/Gene Expression Core provides DHRC investigators with a complementary set of state-of-the-art services and technologies and an integrated framework for analysis of patterns of gene expression in gastrointestinal cells and tissues at multiple levels. New technologies such as DNA microarray, laser capture microdissection, and real-time PCR have revolutionized our ability to study the expression of thousands of genes in defined subsets of cells and tissues. A robust bioinformatics and statistical infrastructure is necessary to analyze and manage such a rich source of experimental data sets. However, access to many of these newer emerging technologies, instrumentation, and analytical tools has been limited and expensive for individual investigators. By providing cost-effective access and expertise in these emerging technologies the core will enhance individual investigator's research efforts as well as foster new collaborations among DHRC members. The specific aims of the Molecular Biology/Gene expression core are to: 1) Enhance the research capabilities of DHRC members by providing consultation, training, and access to a complementary set of services for analyzing patterns of gene expression in a cost-effective, high-throughput manner; 2) Provide an easily accessible core facility where uniform quality controls can be systematically applied to high throughput determination of levels of gene expression in biological tissues and cells; 3) Develop and validate new assays for quantifying expression of genes in cells and tissues; 4) Provide DHRC investigators with training and expertise in bioinformatics and statistical interpretation of microarray and other gene expression data sets; 5) Facilitate integration of emerging new technologies into DHRC investigators research programs, through lectures and formal consultations on principles and methods of analysis of gene expression in gastrointestinal cells and tissues; 6) Perform ongoing evaluation of the quality, cost-effectiveness, and utility of services provided by the Molecular Biology/Gene Expression Core in relation to the needs of the DHRC membership so that the available resources can be allocated to bring the most benefit to DHRC members.

分子生物学/基因表达核心为DHRC研究人员提供了一套互补的最先进的服务和技术，以及一个综合框架，用于分析多个水平的胃肠道细胞和组织中的基因表达模式。DNA微阵列、激光捕获显微切割和实时PCR等新技术彻底改变了我们研究细胞和组织中数千个基因表达的能力。一个强大的生物信息学和统计基础设施是必要的分析和管理这样一个丰富的实验数据集的来源。然而，获得许多这些新的新兴技术，仪器和分析工具一直是有限的和昂贵的个人调查。通过提供具有成本效益的访问和专业知识， 这些新兴技术的核心将加强个人研究人员的研究工作，以及促进DHRC成员之间的新的合作。分子生物学/基因表达核心的具体目标是：1）通过提供咨询，培训和获得一套互补的服务来提高DHRC成员的研究能力，以便以具有成本效益的高通量方式分析基因表达模式;（二）提供一个易于使用的核心设施，统一的质量控制可以系统地应用于高通量测定生物组织和细胞中的基因表达; 3）开发和验证用于定量细胞和组织中基因表达的新测定法; 4）为DHRC研究人员提供生物信息学和统计解释方面的培训和专业知识， 5）通过关于胃肠细胞和组织中基因表达分析的原理和方法的讲座和正式咨询，促进新兴技术与DHRC研究人员研究计划的整合; 6）对质量、成本效益、分子生物学/基因表达核心提供的服务与DHRC成员的需求有关，以便可以分配可用的资源，为DHRC成员带来最大的利益。