CORE--CELL AND MOLECULAR RESOURCES

分子生物学核心实验室的主要目标是促进和促进当代细胞和分子生物学技术在ucchsc及其附属机构现有糖尿病研究项目中的应用。糖尿病研究人员来自不同的临床和科学背景，包括免疫学、外科、发育生物学、生物化学和细胞/分子生物学。虽然大多数人都认识到分子技术在各自领域的价值和影响，但对一些人来说，入门的初始成本和技术本身迅速变化的性质是这些技术应用的主要障碍。因此，该中心将提供“分子住宿和早餐”培训设施，让这些个人及其工作人员能够在与他们感兴趣的项目中获得实际操作的基本技能。对于分子爱好者，Core提供了一个集中的资源，用于自动DNA测序、基因分型、定量PCR、腺病毒和杆状病毒表达系统的开发以及提供质量控制试剂，包括培养基、胰岛cDNA文库、特殊需要载体、胰岛细胞标记物、分子信标、DNA引物和胰腺特异性启动子。将设立咨询服务和公告栏，以提供高级培训，并促进和促进非洲发展委员会成员之间的跨学科和合作企业。该核心将位于芭芭拉戴维斯中心的一个“湿”实验室和设备室，由2FTE技术人员在J. Hutton博士和L. Sussel博士的指导下操作。在很大程度上，它将使用最近从美国国立卫生研究院和慈善基金获得的主要设备。

The principal objective of the Molecular Biology Core Laboratory is to facilitate and promote the application of contemporary cellular and molecular biological techniques to existing diabetes research programs at UCHSC and affiliated institutions. Diabetes researchers come from diverse clinical and scientific backgrounds including immunology, surgery, developmental biology, biochemistry, and cellular/molecular biology. While most recognize the value and impact of molecular techniques on their field, for some the initial cost of getting started and the rapidly changing nature of the technology itself presents a major barrier to application of these techniques. The Core will therefore provide a "Molecular Bed and Breakfast" training facility in which such individuals and their staff can gain hands-on basic skills working on projects specifically related to their interests. For the molecular afficionados , the Core offers a centralized resource for automated DNA sequencing, genotyping, quantitative PCR, development of adenovirus and baculovirus expression systems and provision of quality controlled regents, including media, islet cDNA libraries, special need vectors, islet cell markers, molecular beacons, DNA primers and pancreas-specific promoters. A consultation service and bulletin board will be generated to provide advanced training and to promote and foster interdisciplinary and collaborative ventures among DERC members. The Core will be located in a "wet" laboratory and equipment room in the Barbara Davis Center and manned by 2FTE technical staff under the direction of Dr. J. Hutton and Dr. L. Sussel. For the most part it will use major items of equipment recently obtained from NIH and charitable funding.