Application of high-resolution phosphoproteomics to reveal novel metabolic control mechanisms in Campylobacter jejuni

空肠弯曲菌是西方国家食源性胃肠炎最常见的致病菌。人类感染是由于食用了受污染的鸡肉，近年来发病率有所上升，包括出现了多重耐药弯曲杆菌。需要新的干预措施来减少食物链中的细菌数量。我们知道，细菌中的大多数必需蛋白质都是通过共价修饰来控制其结构的。本项目将试图了解蛋白磷酸化在调节C代谢中的作用。空肠使用尖端蛋白质组学分析，采用高分辨率质谱结合蛋白质功能的生化分析。我们的方法可能会让抗微生物药物或其他干预措施的新目标，以减少弯曲杆菌病的负担。这个跨学科的项目将提供分子微生物学技术的培训，包括多克隆构建和全球蛋白质表达分析，以及蛋白质组学技术在重要生物学问题中的应用。

Campylobacter jejuni is the most common cause of food-borne gastroenteritis inthe western world. Human infections result from consumption of contaminatedchicken and the incidence has increased in recent years, including theemergence of multi-drug resistant campylobacters. New interventions areneeded to reduce the numbers of the bacteria in the food-chain. We know thatmay essential proteins in bacteria are controlled by covalent modifications totheir structure. This project will seek to understand the role of proteinphosphorylation in the regulation of metabolism of C. jejuni using cutting-edgeproteomics analyses employing high-resolution mass spectrometry combinedwith biochemical analysis of protein function. Our approach may allow theidentification of new targets for anti-microbial drugs or other interventions toreduce the burden of Campylobacteriosis. This interdisciplinary project willprovide training in molecular microbiological techniques including mutantconstruction and global protein expression analysis, as well as the application ofproteomic techniques to an important biological problem.