Core 2: Analytical Core

摘要-分析核心 分析核心将作为深层系统级分子表征的中心资源， 由项目和生物样本核心产生的生物样本。这项工作的主要目的是 核心是整合不同的单细胞，批量和空间分析平台，以定义复杂的相互作用 脑肿瘤细胞和相关的脑肿瘤微环境（TME）之间的关系。为此我们 开发了一个分析平台，包括单细胞、单细胞核和大量的转录谱分析。 组织规模;蛋白质表达谱，磷酸化蛋白质组学和免疫肽组学与细胞类型特异性 高空间分辨率的定量代谢组学和药物分布分析 光谱成像（MSI）。这些互补的分析方法具有必要的敏感性， 从单芯活检产生10-20微米厚组织的超深系统级分子谱; 我们平台的性能已经在多个图像引导核心活检中得到了证明， GBM肿瘤的地理上不同的区域。在我们的分析核心中获得的多组学数据将被管理 使用符合FAIR的元数据管理工具，该工具对样本之间的连接进行优先级排序，并定义 离散的样品类型，可以自由连接，如我们的建议书的总体部分所述。的 分析核心将提供专家人员，促进项目内的知识共享，并维护 为这两个项目生成数据所需的先进设备。通过充当公共资源， Analytical Core为每个项目提供专业知识。

ABSTRACT – ANALYTICAL CORE The Analytical Core will serve as a central resource for the deep systems-level molecular characterization of biological samples generated by the Projects and from the Biospecimens Core. The primary objective of this Core is to integrate diverse single cell, bulk, and spatial analytical platforms to define the complex interactions between brain tumor cells and the associated brain tumor microenvironment (TME). To this end, we have developed an analytical platform encompassing transcriptional profiling at the single cell, single nuclei, and bulk tissue scale; protein expression profiling, phosphoproteomics and immunopeptidomics with cell type-specific resolution; and quantitative metabolomics and drug distribution profiling with high spatial resolution by mass spectrometry imaging (MSI). These complementary analytical approaches have the requisite sensitivity to generate ultra-deep systems-level molecular profiling of 10-20 micron thick tissues from single core biopsies; performance of our platform has already been demonstrated on multiple image-guided core biopsies from geographically distinct regions of a GBM tumor. Multi-omics data acquired in our Analytical Core will be managed using a FAIR compliant metadata management tool that prioritizes connections between samples and defines discrete sample types that can be freely connected as described in the Overall Section of our proposal. The Analytical Core will provide expert personnel, facilitate the sharing of knowledge within the program, and maintain the sophisticated equipment necessary to generate data for both Projects. By acting as a common resource, the Analytical Core provides expertise for each Project.