Core 2: Analytical Core

抽象分析核心 分析核心将作为深系统级分子表征的中心资源 由这些项目产生的生物样本和来自生物圈核心的生物样本。这样做的主要目标是 核心是集成不同的单细胞、批量和空间分析平台来定义复杂的相互作用 脑瘤细胞与相关脑瘤微环境(TME)之间的关系。为此，我们有 开发了一个分析平台，包括单细胞、单核和大宗的转录图谱 组织规模；特定细胞类型的蛋白质表达谱、磷蛋白质组学和免疫表位学 以及高空间分辨率的定量代谢组学和药物分布图谱 光谱成像(MSI)。这些互补的分析方法具有必要的敏感度 从单核活检中产生10-20微米厚组织的超深系统级分子图谱； 我们的平台的性能已经在多个图像引导的核心活检上得到了演示 基底膜肿瘤的不同地理区域。在我们的分析核心中获得的多组学数据将得到管理 使用符合公平标准的元数据管理工具来确定样本和定义之间的连接的优先顺序 可以自由连接的离散样本类型，如我们建议书的总体部分中所述。这个 分析核心将提供专家人员，促进计划内的知识共享，并保持 为这两个项目生成数据所需的复杂设备。通过充当公共资源， 分析核心为每个项目提供专业知识。

ABSTRACT – ANALYTICAL CORE The Analytical Core will serve as a central resource for the deep systems-level molecular characterization of biological samples generated by the Projects and from the Biospecimens Core. The primary objective of this Core is to integrate diverse single cell, bulk, and spatial analytical platforms to define the complex interactions between brain tumor cells and the associated brain tumor microenvironment (TME). To this end, we have developed an analytical platform encompassing transcriptional profiling at the single cell, single nuclei, and bulk tissue scale; protein expression profiling, phosphoproteomics and immunopeptidomics with cell type-specific resolution; and quantitative metabolomics and drug distribution profiling with high spatial resolution by mass spectrometry imaging (MSI). These complementary analytical approaches have the requisite sensitivity to generate ultra-deep systems-level molecular profiling of 10-20 micron thick tissues from single core biopsies; performance of our platform has already been demonstrated on multiple image-guided core biopsies from geographically distinct regions of a GBM tumor. Multi-omics data acquired in our Analytical Core will be managed using a FAIR compliant metadata management tool that prioritizes connections between samples and defines discrete sample types that can be freely connected as described in the Overall Section of our proposal. The Analytical Core will provide expert personnel, facilitate the sharing of knowledge within the program, and maintain the sophisticated equipment necessary to generate data for both Projects. By acting as a common resource, the Analytical Core provides expertise for each Project.