DRUG ABUSE RESEARCH CENTER IN MOLECULAR AND CELL BIOLOGY

为了使两个现有的计划项目赠款同步，我们建议 在基础分子和细胞领域建立药物滥用研究中心 明尼苏达大学的生物学，它利用 药理学系核心教师与更有效的推广 与其他药物滥用研究人员的服务和互动 社区。在过去的8年里，我们已经证明了两项P01拨款 作为一个单独的项目，与贺拉斯成功而连贯地合作 因此，在目前的建议中，我们综合了多项 这两个计划项目的组成部分，以建立拟议的药物 虐待研究中心。拟建中心的主要目标是：(A) 促进在药物滥用研究中采用跨学科方法 调查员；(B)充当“活动”中心，协调和 推动所有有关药物滥用研究的学术和学术活动 明尼阿波利斯大都市区；(C)作为国家资源 药物滥用机制的分子和细胞研究 提供专业技术、试剂、克隆细胞系或转基因/基因敲除 动物到地方和全国的药物滥用社区；和(D) 作为明尼苏达州年轻科学家的培训中心。在 拟议的中心，将有一个行政核心，一个 分子/细胞和遗传核心，抗体生产核心和 支持科学研究活动的生物成像核心 组件。共有8个科学组成部分和5个组成部分 它们直接涉及阿片剂的不同方面，而 其他三项涉及其他滥用药物，大麻，尼古丁和 咖啡因。所有这8个组成部分都涉及对基本情况的调查 这些药物滥用的分子和细胞生物学机制。这个 使用不同的方法：转基因动物、分子生物学 蛋白质和基因的突变分析，第二信使规则， 电生理测量和转录控制研究将 为培养青年科学家、互动提供了肥沃的土壤 在调查人员中为药物滥用研究开发资源 当地和全国的社区。

In order to synchronize the two exiting program project grants, e propose to establish a Drug Abuse Research Center in Basic Molecular and Cell Biology at University of Minnesota which utilizes the expertise of the core faculty in the Department of Pharmacology and more effective promotes service and interaction with other drug abuse researchers in this community. During the past 8 years, we have proven that two P01 grants have worked successfully and coherently as a single program, with Horace H. Loh as the P.I. Thus, in the current proposal, we have combined various components from these two program projects to establish the proposed Drug Abuse Research Center. The main objectives of the proposed center are: (a) foster interdisciplinary approaches in drug abuse research among the investigators; (b) to serve as an "activity" center to coordinate and to promote all academic and scholarly activities in drug abuse research in the Minneapolis metropolitan area; (c) to serve as a national resource for molecular and cellular studies in the mechanisms of drug abuse either by providing expertise, reagents, clonal cell lines or transgenic/knockout animals to the drug abuse community locally and nationally; and (d) to serve as training center for young scientists here in Minnesota. Within the proposed center, there will be an Administration Core, a Molecular/Cellular and Genetic Core, an Antibodies Production Core and a Bioimaging Core which will support the activities of the scientific components. There are total 8 scientific components with 5 components which deal directly with the different aspects of the drug opiate, while the other three deals with other drugs of abuse, marijuana, nicotine and caffeine. All these 8 components deal with the investigation of the basic molecular and cell biological mechanisms of these drugs of abuse. The different approaches used: transgenic animals, molecular biological mutational analysis of proteins and genes, second messengers regulations, electrophysiological measurements and transcription control studies will provide a fertile ground for training of young scientists, interaction among investigators nd developing resource for the drug abuse research community locally and nationally.