Cloning of a Hookworm Excretory-Secretory Protein

描述：候选人提供)：钩虫是一种主要的全球公共 健康问题，感染了全球超过10亿人。吸血成虫 钩虫是发育中贫血和营养不良的主要原因 对儿童和妇女造成了特别毁灭性的损失 生育年龄。成虫在体内分泌多种因子 肠道中可能导致疾病的附着 发病机制。因此，这个项目的最初目标是分子 新基因ACES-1的克隆、表达和鉴定 从人钩虫中分离出的排泄物分泌蛋白 塞兰钩口线虫。利用钩虫感染动物模型研究钩虫感染的作用 然后将使用主动和被动来检查发病机制中的ACE-1 免疫技术。除了散发出自然界的光芒 宿主-寄生虫的相互作用，对ACE-1等因子的研究最终可能会产生 免疫或药物干预的新靶点，以减少 钩虫病的负担。

DESCRIPTION: provided by the candidate): Hookworms are a major global public health problem, infecting over a billion people worldwide. Blood feeding adult hookworms are a leading cause of anemia and malnutrition in developing countries, extracting a particularly devastating toll on children and women of childbearing age. Adult worms secrete numerous factors at the site of attachment in the intestine that are likely to contribute to disease pathogenesis. Accordingly, the initial aims of this project are the molecular cloning, expression, and characterization of AcES-1, a novel excretory-secretory protein which ahs been isolated from the human hookworm Ancylostoma ceylanicum. Using an animal model of hookworm infection the role of AcES-1 in pathogenesis will then be examined using active and passive immunization techniques. Aside from shedding light in the nature of the host-parasite interaction, study of factors such as AcES-1 may ultimately yield novel targets for immunological or pharmacological intervention to reduce the burden of hookworm disease.