NPIF: Cultured plant cells as platforms for the production of high value chemicals

该项目将涉及采用最先进的植物细胞培养方法，从一种植物物种中分离培养的植物细胞并随后对其进行表征，该植物物种是给定高价值化学品的来源。分离的植物细胞随后将通过显微镜、生物化学和基于转录组学的方法进行表征。然后将采用基于合成生物学的程序来增加从分离的植物细胞系中产生目标化学品。 BBSRC在其战略计划中将工业生物技术确定为一个高级别战略优先领域，迫切需要在这一领域进行能力建设。此外，BBSRC旨在支持开发新的可持续方法，以使用可再生资源和利用生物过程产生化学品。该项目将直接解决BBSRC的这些优先事项。该项目还将涉及与英国工业界的直接合作，使基本发现最终转化为新产品和新工艺。BBSRC的战略计划也强调了这些目标。BBSRC的其他优先事项是支持应用全细胞系统生产高价值化学品的新方法，特别是那些利用合成生物学方法实现这些目标的方法。同样，我们的项目完全符合BBSRC的这些目标。 总的来说，该项目将通过开发可持续的基于细胞的系统，利用合成生物学程序，生产高价值的植物化学品，提供具有影响力的尖端科学。

The project will involve the isolation and subsequent characterisation of cultured plant cells from a plant species which is the source of the given high value chemical employing state-of-the-art plant cell culture methods. The isolated plant cells will subsequently be characterised by microscopic, biochemical and transcriptomics-based approaches. Synthetic biology based procedures will then be employed to increase production of the target chemical from the isolated plant cell line. BBSRC has identified industrial biotechnology in its Strategic Plan as a high-level strategic priority area, with an urgent need to build capacity and capability in this area. Further, BBSRC aims to support the development of new sustainable approaches to the generation of chemicals using renewable resources and utilising biological processes. This project will directly address these BBSRC priorities. This project will also involve direct collaboration with UK industry, allowing the translation of basic discoveries ultimately into new products and processes. These goals are also highlighted in BBSRC's Strategic Plan. Additional BBSRC priorities are to support new approaches for the application of whole cell systems for the production of high-value chemicals, especially those utilizing synthetic biology approaches to reach these goals. Again, our project fits perfectly with these BBSRC goals. In aggregate, this project will deliver cutting-edge science with impact, by developing a sustainable cell based system, utilizing synthetic biology procedures, for the production of a high value plant chemical.