Gene Expression and Discovery in Liver and Gut Stem Cel*

描述（由申请人提供）： 本申请是对标题为NIDDK祖细胞基因组项目（RFA-DK-02-027）的RFA的回应。该项目将协调几名具有干细胞分离（Goodell）、肝细胞生物学（达林顿，韦斯）、肠道发育（Henning）、生物统计学（Shaw）、cDNA微阵列制造和分析（达林顿，白色）、基于新序列策略的基因谱分析（Gunaratne）以及数据库管理和数据分发（Steffen，Shaw）专业知识的研究人员。除了韦斯博士外，这些研究人员都是贝勒医学院的教员。本申请的目的是表征来自肝脏、肠的推定干细胞群和从胚胎肝脏获得的细胞群，这些细胞群能够在培养物中维持并显示出体外分化的能力。这些细胞的表征将以五种方式进行：（1）通过分析与造血谱系中的干细胞相关的已知标志物，（2）通过分析成熟肝或肠细胞的标志物，（3）通过使用富马酰乙酰乙酸水解（FAH）缺陷小鼠移植至肝脏，（4）通过cDNA微阵列分析，和（5）通过基于序列的基因分析方法。将通过一个网站，利用便于研究界查阅的软件和界面，对这些特征描述所产生的数据进行整理和分发。方案、定制阵列和细胞系将根据要求分发。

DESCRIPTION (provided by applicant): This application is sent in response to the RFA entitled NIDDK Progenitor Cell Genome Projects (RFA-DK-02-027). The Project will coordinate several investigators with expertise in stem cell isolation (Goodell), liver cell biology (Darlington, Weiss), intestinal development (Henning), biostatistics (Shaw), cDNA microarray manufacture and analysis (Darlington, White), gene profiling by a novel sequence based strategy (Gunaratne), and database management and data distribution (Steffen, Shaw). With the exception of Dr. Weiss, these investigators are faculty at Baylor College of Medicine. The aims of the application are to characterize populations of putative stem cells from the liver, intestine and cells obtained from embryonic liver that are able to be maintained in culture and that show the capacity to differentiate in vitro. Characterization of these cells will be done in five ways; (1) by analysis of known markers that are associated with stem cells in the hematopoietic lineage, (2) by analysis of markers of mature liver or gut cells, (3) by transplantation to the liver using the fumarylacetoacetate hydrolyze (FAH) deficient mouse, (4) by cDNA microarray analysis, and (5) by a sequence based gene profiling approach. The data from these characterizations will be organized and distributed through a web site using software and interfaces that will permit easy access by the research community. Protocols, custom arrays and cell lines will be distributed on request.