MICROBIOTA OF CHILDREN WITH ORAL HEALTH DISPARITIES

这项建议的长期目标是为设计治疗方案提供可靠的科学基础，以改善口腔健康差距的儿童和青少年的牙齿健康。当前项目的目标是检查与儿童龋齿相关的口腔微生物区系，寻找与儿童和青少年疾病增加有关并可以解释的差异，以及种族和社会经济群体在人口口腔健康差异中的差异。将监测一系列可能与龋病相关的物种，包括变形链球菌、几种放线菌和双歧杆菌、物种和选定的牙周病原体。第一个目的是使用DNA探针棋盘试验评估幼儿和青少年的微生物区系。将评估不同年龄、种族或民族以及社会经济群体中微生物种类与龋病的相关性。儿童时期口腔微生物区系的发育顺序在有无疾病的儿童中可能有所不同。有口腔健康差距的成年人的口腔健康不良可能是基于并可以从儿童和青春期的微生物区系预测的。第二个目标是将龋齿的检测从牙科办公室转移到儿科医生。一种简单的微生物采样方法将被评估其改善儿童早期龋齿检测的能力。第三个目的是评估严重的儿童早期龋齿的微生物区系，这是儿童中最普遍的口腔感染，可能影响儿童的总体健康。其目的是评估综合治疗是否将微生物区系改变为无龋齿儿童的微生物区系，以及治疗后对包括变形链球菌在内的龋病相关物种的抑制如何与治疗成功相关。来自该项目的信息应能阐明与儿童龋齿有关的微生物区系。在临床上具有重要意义的是，该项目通过评估改进的儿童龋齿诊断和风险评估，寻求促进改善未得到充分服务的人群的牙齿健康的方法，以便有效的治疗可以针对最需要帮助的儿童和青少年。

The long-term goal of this proposal is to provide a sound scientific basis on which to devise therapy regimens that can improve dental health of children and adolescents with oral health disparities. The objective of the current project is to examine the oral microbiota associated with dental caries in children, seeking differences that associate with and could explain increase d disease in children and adolescents, and differences in racial and socioeconomic groups in a population oral health disparities. A range of possible caries-associated species will be monitored including the mutans streptococci, several Actinomyces and Bifidobacterium, species, and selected periodontal pathogens. The first aim evaluates the microbiota of young children and adolescents using a DNA probe checkerboard assay. The association of microbial species with caries in different age, racial or ethnic, and socioeconomic groups, will be evaluated. The sequence of development of the oral microbiota in childhood may differ in children with and without disease. It is possible that poor oral health of adults with oral health disparities is based on, and could be predicted from the microbiota of childhood and adolescence. The second aim takes detection of caries out of the dental office into that of the pediatrician. A simple microbial sampling method will be evaluated for its ability to improve detection of early childhood caries. The third aim evaluates the microbiota of severe early childhood caries, the most prevalent oral infection in children that can impact children's general health. The aim evaluates whether comprehensive therapy changes the microbiota to that of caries-free children, and how the suppression of caries-associated species, including Streptococcus mutans, post-therapy, are associated with success of treatment. Information from this project should clarify the microbiota associated with childhood caries. OF clinical importance this project seeks ways facilitate improvement of dental health of under-served populations directed by evaluating improved diagnosis and risk assessment of childhood caries so effective therapy can be directed to children and adolescents most in need.