MICROBIAL RISK FACTORS FOR DENTAL IMPLANTS

尽管骨结合的牙种植体通常成功率很高 大约10%的植入物失败。这项提案的总体目标 识别在种植失败中起作用的微生物风险因素，以及 评估抗菌治疗在预防和治疗中的潜力 牙种植体失败的治疗。 第一个具体目标包括一个主要的可培养的失败研究。 牙齿植入物。骨整合成功与失败的微生物区系 植入物将被比较。植入物将以临床为特征 使用标准化X光照片进行测量、附件级别、机动性、 以及炎症，包括种植体周围的温度测量。 将使用荧光底物测试来表征微生物 生化反应和聚丙烯酰胺凝胶电泳法。分离物 将通过将特征与参考特征进行比较来确定 种的品系，包括新的和重新分类的种。猪瘟病毒分离株 未知物种将通过rRNA测序和DNA探针进行表征。 第二个具体目标是评估抗菌药物的潜力。 治疗失败种植体的方法。失败的植入物将被治疗 使用一种非手术、积极的抗菌疗法。植入物将是 使用从第一个特定目标开始的临床测量进行监测。 治疗成功的标志是骨密度和/或 身高使用减影X光照相。治疗后的微生物区系 治疗后的植入物将使用主要的培养方法进行表征。 和第一个目标一样。不同药剂处理前后微生物区系的比较 从成功和未成功处理的植入物中获得的样本将 提供更多数据以区分与 植入失败。 其长期目标是提高种植牙的成功率。 本提案中的信息将有助于确定具体的 失败植入物的治疗方案。其目标是确定 调节、指示或预测植入成功的微生物或 失败了。然后可能会制定预防措施，以改善 种植成功的概率。

Although osseointegrated dental implants generally have a high success rate, about 10% of implants fail. The broad objectives of this proposal are to identify microbial risk factors operative in implant failure, and assess the potential for antibacterial therapy in the prevention and treatment of failing dental implants. The first specific aim includes a predominant cultivable study of failing dental implants. The microbiotas of successful and failing osseointegrated implants will be compared. Implants will be Characterized by clinical measurements using standardized radiographs, attachment level, mobility, and inflammation including peri-implant temperature measurements. Microorganisms will be characterized using fluorescent substrate tests for biochemical reactivity, and polyacrylamide gel electrophoresis. Isolates will be identified by comparison of characteristics with those of reference strains of species, including new and reclassified species. Isolates of unknown species will be characterized by rRNA sequencing and DNA probes. The second specific aim will assess the potential of antibacterial treatment to treat failing Implants. Failing implants will be treated using a non-surgical, aggressive antimicrobial therapy. Implants will be monitored using the clinical measurements from the first specific aim. Successful treatment will be indicated by increases in bone density and/or height using subtraction radiography. The post-treatment microbiota of treated implants will be characterized using predominant cultural methods as in the first aim. Comparison of pre- and post-treatment microbiota from samples obtained from successfully and unsuccessfully treated implants will provide additional data to differentiate species associated with implant-failure. The long-term objective is to improve the success-rate of dental implants. Information from this proposal will contribute to determining specific treatment regimens for failing implants. The objective is to identify microorganisms that mediate, indicate or predict for implant success or failure. Preventive measures may then be devised to improve the probability of dental implant success.