Microarray Center for Research on the Nervous System

描述(申请人提供)：本研究的基因芯片中心 儿童遗传医学中心？S国家医学中心，华盛顿特区 提供所附的建议书，其中NINDS/NIMH微阵列的概要 描述了用于表达谱分析的中心(MC)。为实验提供帮助 将提供设计、数据生成和数据解释。它是 重要的是要认识到，儿童？S基因芯片中心已经实现了 为NHLBI、NEI和许多其他调查人员提供的这些功能，实际上已经 完善的实验协议、网站和创新 生物信息学方法，允许以相对较高的吞吐量 低成本(由于与其他赠款和方案分担费用)。简而言之，MC 是独一无二的，因为我们已经建立了一个表情分析中心，并积累了 通过1)NHLBI PGA大规模表达奖获得重要专业知识 简介(全国仅有的11个之一)和NEI关于cdna的内部合同 建设，2)成为Affymetrix技术的“卓越中心”， 3)开发高度集成的阵列网站 (microarray.cnmcResearch.org)用于教程和数据传播，4) 我国第一个基于Web的Oracle数据查询算法的开发 存储库，5)精通cDNA和Affymetrix技术，6) 进行了超过975次Affymetrix阵列实验，7)MC的操作 作为一家拥有常驻基础设施的企业，8)为50多名访客提供帮助 到目前为止，拥有阵列实验的调查人员，以及常驻科学家 为实验设计提供帮助的专业知识。在本次发售中，我们建议 建立NINDS/NIMH表达谱芯片中心 现有儿童？S基因芯片中心。大部分拟议的结构和 MC的功能是基于我们以前使用类似 NHLBI资助的拨款。MC的目标包括：1)基于Web的协议 教程和提交，2)通过实验方案进行审查和修订 具有丰富神经科学经验的委员会(EPC)，3) 调查人员驱动的按存储容量使用计费机制(3级补贴)，4)数据 生成，5)Oracle仓储，自动定时向公众发布 通过我们网站的网络访问和NCBI，6)我们数据存储库的网络查询，5) 数据分析教程和帮助，以及6)开发定制Affymetrix NeuroChips使用我们的下游大型数据结构和我们的 作为一种机制，开发一种独特的、低成本的试剂，用于 社区。虽然我们精通斑点DNA阵列和Affymetrix 阵列，我们计划提供Affymetrix股票芯片作为主要数据生成 NINDS/NIMH调查平台。这是因为固有的 可移植性和数据库结果的能力，允许所有 调查人员使用所有其他调查人员的结果。另外， ORACLE/LIMS中核心数据库结构的数据库处理和Web访问 Affymetrix平台正在变得相当成熟，而这种数据库和Web 使用斑点cdna阵列的Access要成熟得多。我们已经印制了 并用cDNA阵列(特别是小鼠BMAP)进行了大型实验 SET)，该平台将向NINDS/NIMH资助的调查人员提供 一项有限的可选服务，即使大多数基因 在BMAP集合中也在MG_U74阵列上(超过74%)。

DESCRIPTION (provided by applicant): The Microarray Center of the Research Center of Genetic Medicine, Children?s National Medical Center, Washington DC offers the attached proposal, wherein an outline of a NINDS/NIMH Microarray Center (MC) for expression profiling is described. Assistance with experimental design, data generation, and data interpretation will be provided. It is important to recognize that the Children?s Microarray Center already fulfills such functions for NHLBI, NEI, and many other investigators, and, indeed, has well-established experimental protocols, web sites, and innovative bioinformatics methods which permit a high degree of throughput at relatively low cost (due to cost sharing with other grants and programs). Briefly, the MC is unique because we have built an expression profiling Center and amassed significant expertise through 1) an NHLBI PGA award for large-scale expression profiling (one of only 11 nationally) and an NEI intramural contract for cDNA construction, 2) becoming a "Center of Excellence" for Affymetrix technology, 3) development of a highly integrated array web site (microarray.cnmcresearch.org) for tutorials and data dissemination, 4) development of the first web-based query algorithm of our Oracle data repository, 5) being well-versed in both cDNA and Affymetrix technology, 6) having performed over 975 Affymetrix array experiments, 7) operation of the MC as a business with resident infrastructure, 8) assisting over 50 visiting investigators with array experiments to date, and 9) having resident scientific expertise for experimental design assistance. In this offering, we propose to establish a NINDS/NIMH Microarray Center for expression profiling within the existing Children?s Microarray Center. Much of the proposed structure and function of the MC is based upon our previous experience with a similar NHLBI-funded grant. The goals for the MC include: 1) web-based protocol tutorials and submission, 2) review and revisions by an experimental protocol committee (EPC) which has extensive neuroscience experience, 3) investigator-driven charge-back mechanisms (3 levels of subsidization), 4) data generation, 5) Oracle warehousing with automated timed release to the public via web-access at our site and NCBI, 6) web queries of our data repository, 5) data analysis tutorials and assistance, and 6) development of custom Affymetrix NeuroChips using our downstream large data structure and our "Center of Excellence" status as mechanisms to develop a unique, low-cost reagent for the community. While we are adept at both spotted cDNA arrays and Affymetrix arrays, we plan to offer Affymetrix stock chips as the primary data generation platform for NINDS/NIMH investigators. This is because of the inherent transportability and the ability to database results, allowing all investigators to use the results of all other investigators. Also, the databasing and web access to kernal database structures in the Oracle/LIMS Affymetrix platform is becoming quite mature, while such databasing and web access is considerably less mature with spotted cDNA arrays. We have printed and conducted large experiments with cDNA arrays (particularly the murine BMAP set), and this platform will be available to NINDS/NIMH-funded investigators as an optional service at limited, availability, even though the majority of genes in the BMAP collection are also on the MG_U74 array (more than 74%).