MULTIDAY EXPOSURE TO O3 IN ASTHMA

描述：（改编自研究者摘要） 本申请人实验室建议哮喘患者具有增强的 炎症反应短期吸入臭氧。 不知道， 然而，是否有进展的炎症反应， 反复短期暴露的哮喘患者（如 许多烟雾事件）。 臭氧引起的人体炎症的进展 与哮喘可能会增加急性加重的风险， 疾病 大多数数据表明，臭氧诱导呼吸道 健康和哮喘受试者的气道炎症产生于 涉及支气管镜检查和支气管肺泡灌洗（BAL）分析的研究 液 在分析诱导痰是否已被验证用于 调查吸入性过敏原的影响，它还没有得到充分的 用于空气污染研究。 事实上， 在臭氧暴露研究中使用该技术表明， 痰液（对近端气道进行采样）可能会产生冲突的结果， 分析BAL液（对远端肺进行采样）。 为了 验证痰诱导（SI）在以下研究中的应用 臭氧引起的呼吸道炎症，并评估 哮喘患者反复短期接触臭氧，三项研究是 提出了 研究1旨在直接比较诱导的 哮喘患者短期臭氧暴露后痰液对支气管肺泡灌洗液影响 科目 研究2的目的是比较从痰液中获得的结果 诱导和支气管镜检查后，反复短期暴露，以确定 近端气道和远端气道的炎症效应是否不同 肺。 研究3将检验以下假设： 哮喘患者中臭氧诱导的呼吸道炎症 单独使用痰液诱导或同时使用痰液诱导的重复短期暴露 痰诱导和支气管镜检查，根据前两个结果 问题研究 预计拟议的研究将有助于验证 空气污染研究中的痰诱导问题，并解决 增加哮喘患者对急诊室和医院的利用率 暴露于臭氧是由于与增强气道有关的急性加重 炎症

DESCRIPTION: (Adapted from the Investigator's Abstract) Recent data from the applicant's laboratory suggest that persons with asthma have an enhanced inflammatory response to short-term inhalation of ozone. It is not known, however, whether there is progression of the inflammatory response in asthmatic persons with repeated short-term exposures (as would occur during many smog episodes). Progression of ozone-induced inflammation in persons with asthma is likely to increase the risk of acute exacerbation of the disease. Most of the data demonstrating that ozone induces respiratory tract inflammation in healthy and asthmatic subjects has been generated from studies involving bronchoscopy and analysis of bronchoalveolar lavage (BAL) fluid. While analysis if induced sputum has been validated for the investigation of effects of inhaled allergen, it has not been adequately validated for use in air pollution studies. In fact, early reports of the use of the technique in ozone exposure studies suggest that analysis for sputum (which samples the proximal airways) may yield results that conflict with analysis of BAL fluid (which samples the distal lung). In order to validate the use of sputum induction (SI) in the investigation of ozone-induced respiratory tract inflammation and to asses the effects of repeated short-term ozone exposures in asthmatic subjects, three studies are proposed. Study 1 is designed to directly compare analysis of induced sputum to that of BAL fluid after short-term ozone exposure in asthmatic subjects. Study 2 is designed to compare the results obtained from sputum induction and bronchoscopy after repeated short-term exposures to determine whether inflammatory effects are different in proximal airways and distal lung. Study 3 will test the hypothesis that there is a progression of ozone-induced respiratory tract inflammation in asthmatic subjects with repeated short-term exposure using either sputum induction alone or both sputum induction and bronchoscopy, depending on the results of the first two studies. It is anticipated that proposed research will help to validate the issue of sputum induction in air pollution studies and address whether the increased emergency department and hospital utilization of asthmatic persons exposed to ozone is due to acute exacerbation related to enhanced airway inflammation.