Effects of multiday exposure to ozone on airway inflammation as determined using sputum induction.
Effects of multiday exposure to ozone on airway inflammation as determined using sputum induction.
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使用痰诱导确定的多天日暴露于臭氧对气道炎症的影响。
DOI：
10.1289/ehp.8341
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发表时间：
2006-02
影响因子：
10.4
通讯作者：
Balmes J
中科院分区：
文献类型：
--
作者：
Ratto J;Wong H;Liu J;Fahy J;Boushey H;Solomon C;Balmes J
已知单次短期暴露于臭氧会导致肺功能的急性变化和中性粒细胞性气道炎症。反复接触对呼吸系统健康的影响尚未得到充分研究。众所周知，肺功能下降会减弱，但尚不清楚损伤和炎症是如何受到影响的。我们设计了一项研究，利用痰诱导 (SI) 对单日和多日暴露后的呼吸道内壁液体进行采样，以检验多日暴露后中性粒细胞与单日暴露相比会增加的假设。在随机交叉设计中，15 名正常健康受试者在两种条件下暴露于 O3 (0.2 ppm)：1 天 4 小时 (1D) 和连续 4 天 4 小时 (4D)。每 4 小时暴露之前和之后立即进行肺功能测试。 SI 是在 1D 和 4D 条件结束后 18 小时进行的。症状和肺功能数据遵循其他多日氧气暴露研究中的模式，最大的变化发生在第二天。然而，与之前使用支气管肺泡灌洗的研究相比，与 1D 条件相比，4D 条件后中性粒细胞的百分比显着增加，巨噬细胞的百分比显着下降。鉴于 SI 可能比远端肺更好地采样近端气道，这些结果进一步证明人类和其他物种对 O3 的气道区室反应存在差异。
Single short-term exposures to ozone are known to cause acute changes in pulmonary function and neutrophilic airway inflammation. The respiratory health effects of repeated exposures are not as well studied. Pulmonary function decrements are known to attenuate, but it is less clear how injury and inflammation are affected. Using sputum induction (SI) to sample respiratory tract lining fluid after single- and multiday exposures, we designed a study to test the hypothesis that neutrophils would increase after multiday exposure compared with single-day exposure. In a randomized, crossover design, 15 normal healthy subjects were exposed to O3 (0.2 ppm) under two conditions: for 4 hr for 1 day (1D) and for 4 hr for 4 consecutive days (4D). Pulmonary function testing was performed immediately before and after each 4-hr exposure. The SI was performed 18 hr after the end of the 1D and 4D conditions. The symptom and pulmonary function data followed a pattern seen in other multiday O3 exposure studies, with the greatest changes occurring on the second day. In contrast to previous studies using bronchoalveolar lavage, however, there was a significant increase in the percentage of neutrophils and a significant decrease in the percentage of macrophages after the 4D condition compared with the 1D condition. Given that SI likely samples proximal airways better than distal lung, these results add to the body of evidence that differential airway compartmental responses to O3 occur in humans and other species.
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影响因子：
6
作者：
HILTERMANN, TJN;STOLK, J;DIJKMAN, JH
通讯作者：
DIJKMAN, JH
影响因子：
3.3
作者：
SELTZER, J;BIGBY, BG;BOUSHEY, HA
通讯作者：
BOUSHEY, HA
DOI：
10.1164/ajrccm/138.1.81
发表时间：
1988-07-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
KELLY, CA;FENWICK, JD;WALTERS, EH
通讯作者：
WALTERS, EH
DOI：
10.1165/ajrcmb.14.1.8534488
发表时间：
1996-01-01
影响因子：
6.4
作者：
Duan, XZ;Buckpitt, AR;Plopper, CG
通讯作者：
Plopper, CG
影响因子：
3.3
作者：
HACKNEY, JD;LINN, WS;COLLIER, CR
通讯作者：
COLLIER, CR