STRUCTURE AND FUNCTION OF CYTOPLASMIC DYNEIN

描述(改编自申请人的摘要)：使用以下组合 在果蝇的遗传学和分子生物学中，海斯将研究动力蛋白是如何 靶向不同的组织/细胞以执行各种功能。他会的 专注于动力蛋白中间体和轻链，因为它们已经 被指示为具有目标功能。他将生成空值，分析 由此产生的表型，也将表征相互作用的蛋白质 有轻链和中间链。他将与动力蛋白一起进行研究 帮助阐明动力蛋白在有丝分裂中的作用。这些也将 涉及神经母细胞不对称分裂的研究。体外协作 研究将涉及动力蛋白与渗透性动素的结合。 系统。由于动力蛋白与货物结合在许多情况下似乎是 在动力蛋白的介导下，改变动力蛋白-动力蛋白相互作用的突变体将 特色化的。这是一项对各种运动的全球研究， 胞质动力蛋白在真核细胞中发挥作用。

DESCRIPTION (Adapted from applicant's abstract): Using a combination of genetics and molecular biology in Drosophila, Hays will study how dynein is targeted to different tissue/cells to perform a variety of functions. He will concentrate on the dynein intermediate and light chains since they have been indicated as having a targeting function. He will generate nulls, analyzing the resulting phenotypes, and will also characterize proteins that interact with the light and intermediate chains. He will carry out studies with dynein mutants to help elucidate the role of dynein in mitosis. These will also involve studies on asymmetric divisions in neuroblasts. In vitro collaborative studies will involve binding of dynein to kinetochores in permeabilized systems. Since dynein binding to cargo appears in many instances to be mediated by dynactin, mutants that modify dynein-dynactin interactions will be characterized. This is a global study of the various motilities that cytoplasmic dynein performs in eukaryotic cells.