REGULATION OF GLUTAMATE SYNTHESIS IN BACILLUS SUBTILIS

该项目的长期目标是在 分子水平上控制细胞合成和活性的机制 芽孢杆菌中将乙酰辅酶A和草酰乙酸酯转化为谷氨酸的酶 枯草杆菌。这条对产生能量至关重要的途径，减少了 电力和生物合成积木也是碳的结合点 和氮代谢，并在细菌中起着关键的调节作用 差异化。这个项目之前的工作导致了这个发现或 七种蛋白质(CCPC、CCPA、Cody、GLTC、GltR、TnrA和RocR)的鉴定 参与编码这些酶的基因的调节。这个 本提案的具体目的是揭示分子机制。 其中几个调控蛋白通过它来控制转录 柠檬酸合酶、乌头酸酶、异柠檬酸脱氢酶、谷氨酸合酶和 谷氨酸脱氢酶基因和发现效应分子 控制某些调节蛋白的活性。枯草杆菌 系统是研究革兰氏阳性菌和 原核细胞分化。枯草杆菌基因的基础研究 表达，新陈代谢的调节和对环境的反应都很高 提供关于相关致病细菌生物学的信息，并提供 研究生物体中具有普遍生物学重要性的问题的手段 在生理上和基因上都很容易被操纵。

The long-term goal of this project is to understand at the molecular level the mechanisms that control synthesis and activity of the enzymes that convert acetyl CoA and oxaloacetate to glutamate in Bacillus subtilis. This pathway, which is critical for generation of energy, reducing power, and biosynthetic building blocks, is also the junction between carbon and nitrogen metabolism and plays a key regulatory role in bacterial differentiation. Previous work on this project has led to the discovery or identification of seven proteins (CcpC, CcpA, CodY, GltC, GltR, TnrA, and RocR) that participate in regulation of the genes that encode these enzymes. The specific aims of the present proposal are to uncover the molecular mechanisms by which several of these regulatory proteins control transcription of the citrate synthase, aconitase, isocitrate dehydrogenase, glutamate synthase, and glutamate dehydrogenase genes and to discover the effector molecules that control the activities of some of the regulatory proteins. The B. subtilis system is the primary paradigm for studies of Gram-positive bacteria and of prokaryotic differentiation. Fundamental studies of B. subtilis gene expression, regulation of metabolism and response to the environment are highly informative about the biology of related pathogenic bacteria and provide a means of studying issues of universal biological importance in an organism that is easily manipulated physiologically and genetically.