MECHANISM OF SPINDLE ASSEMBLY AND CHROMOSOME SEGREGATION

描述：目标1是进一步定义XKCM1如何通过以下方式破坏微管的稳定性 一系列领域研究。第二系列的研究将集中在 建立微管失稳的进化保守性 其他Kini家庭成员的活动。目标2是识别和描述 与XKCM1相互作用的蛋白质。在这里，申请人特别是 有兴趣确定潜在的动粒成分，涉及 XKCM1与染色体的关联。目的3是为了研究其发病机制。 非洲爪哇提取物中的染色体分离。目标4是使用开发的试剂 在以往的研究中，为了在体内干扰XKCMP1的功能。目标5是评估 XKCMP1功能已被操纵的细胞中的染色体运动 先前的目标。

DESCRIPTION: Aim 1 is to further define how XKCM1 destabilizes microtubules by a series of domain studies. A second series of studies will be focused on establishing evolutionary conservation of the microtubule destabilizing activity of other KinI family members. Aim 2 is to identify and characterize proteins that interact with XKCM1. Here the applicant is particularly interested in identifying potential kinetochore components involved in association of XKCM1 with chromosomes. Aim 3 is to investigate the mechanism of chromosome segregation in Xenopus extracts. Aim 4 is to use reagents, developed in previous studies, to disrupt XKCMP1 function in vivo. Aim 5 is to assess chromosome movement in cells where XKCMP1 function has been manipulated as in the previous aim.