Candida albicans oral biofilm

描述(申请人提供)：白色念珠菌是一种共生菌 定植于皮肤和粘膜表面，包括口腔。这种有机体是 也是这些表面和内部的机会性疾病的病原体 播散性疾病。口腔念珠菌病与牙周组织紊乱有关 与新生儿和新生儿获得微生物有关的口腔菌群 抗菌治疗、口腔修复和糖尿病等宿主因素 糖尿病和艾滋病毒感染。口腔表现包括伪膜性 念珠菌病(画眉)和义齿口腔炎。口咽部感染是 艾滋病几乎不可避免的后果(96%的患者)和 经常重复发生。义齿口腔炎可能会影响50%的完全性 戴假牙的人。这种有机体在粘膜、牙齿和口腔器械上形成生物膜 例如假牙，通常与口腔细菌有关。与.相比 浮游细胞、生物膜中的生物具有减少 对抗真菌药物的敏感性和细胞外基质的存在。 这项研究将检验这样一种假设，即独特的特征与 白念珠菌生物膜通常是基因表达改变的结果。 细胞代谢以及生物膜特异性基因的表达。一种模型 将使用本实验室建立的唾液涂层义齿。 大约230种一般细胞代谢的变化已经被确定在 利用生物膜与浮游细胞的高度同源性比较生物膜 酿酒酵母和白色念珠菌及其商品化 酿酒酵母基因芯片。在目标1中，这一方法将应用于 在生物膜形成和其他条件下检测表达的时间性 利用白色念珠菌DNA芯片开发生物膜。在目标2中，在中表达 体外生物多样性模型也将被检验，以确定基因的内在 与生物膜相关，区别于那些受生物膜影响的生物膜 环境。从固有生物膜表达中选择的基因的表达 将在体内确定从人类唾液中提取的生物体的类别。目标 3将研究生物膜调节基因如TUP1和EFG1的作用 转基因菌株。

DESCRIPTION (provided by applicant): Candida albicans is a commensal that colonizes skin and mucosal surfaces including the oral cavity. The organism is also an agent of opportunistic disease of these surfaces as well as internal disseminated disease. Oral candidiasis is associated with derangements of the oral flora related with the acquisition of microbes by neonates and anti-bacterial therapy, oral prostheses, and host factors such as diabetes mellitus and HIV infection. Oral manifestations include pseudomembraneous candidiasis (thrush) and denture stomatitis. Oropharyngeal infection is virtually an inescapable consequence of AIDS (96 percent patients) and frequently reoccurs. Denture stomatitis may affect 50 percent of complete denture wearers. The organism forms biofilms on mucosa, teeth and oral devices such as dentures, generally in association with oral bacteria. Compared to planktonic cells, organisms in biofilms have characteristics such as reduced susceptibility to antifungal drugs and the presence of an extracellular matrix. This study will test the hypothesis that unique characteristics associated with C. albicans biofilms are the result of altered gene expression in general cellular metabolism as well as bioflim specific gene expression. A model of saliva-coated denture acrylic established in this laboratory will be used. About 230 alterations in general cellular metabolism have been identified in biofilm compared to planktonic cells by exploiting the high homology between Saccharomyces cerevisiae and C. albicans and the commercial availability of gene arrays for S. cerevisiae. In Aim 1 this approach will be applied to examine expression temporally during biofilm formation and to other conditions of biofilm development using C. albicans DNA chips. In Aim 2, expression in in vitro biodiverse models will also be examined to identify genes inherently associated with biofilms as differentiated from those influenced by the biofilm environment. Expression of selected genes from the inherent biofilm expression class will be determined in vivo in organisms recovered from human saliva. Aim 3 will examine the role of biofilm-regulated genes such as TUP1and EFG1 using genetically modified strains.