Candida albicans-specific mucosal CD4+ T cell clones

描述（由申请人提供）： 白色念珠菌是一种寄生于皮肤和粘膜表面（包括口腔）的真菌。生物体在粘膜和口腔装置（例如假牙）上形成生物膜，通常与细菌相关。该生物体也是这些表面的机会性感染以及传播疾病的媒介。在最易受口腔感染的个体中，HIV+患者的CD4+ T细胞下降。这种淋巴细胞群体的损失和疾病之间的相关性暗示了CD4+ T细胞在保护健康个体免受疾病的影响。CD4+ T细胞也与感染鼠模型的易感性有关。虽然辅助性T细胞1型细胞介导的免疫（CMI）已经涉及全身和粘膜感染的保护和恢复，但也清楚的是，粘膜和全身免疫应答具有区室化的要素。这是强调了艾滋病患者的易感性，口腔粘膜，而不是全身性疾病。口腔粘膜中T细胞的分布不同于外周血中的分布。近年来，T细胞克隆已成为鉴定刺激CMI应答的特异性抗原及其表位的有用工具。然而，这些协议的应用已被应用于外周循环的淋巴细胞，而不是那些在口腔病原体的反应中很重要的口腔粘膜。本提案的目的是建立分离CD4+ T细胞克隆的程序，这些克隆来源于C定植小鼠口腔粘膜的T细胞群。白色念珠菌该方案的成功开发具有特定的应用，将为后续研究宿主反应和控制口腔念珠菌提供重要工具。白色念珠菌一个成功的方案也将有更广泛的应用，以研究粘膜对正常口腔植物群和口腔感染因子的反应。

DESCRIPTION (provided by applicant): Candida albicans is a commensal that colonizes skin and mucosal surfaces including the oral cavity. The organism forms biofilms on mucosa and oral devices, e.g. dentures, generally in association with bacteria. The organism is also an agent of opportunistic infection of these surfaces as well as disseminated disease. Among individuals that are most susceptible to oral infection are HIV+ patients who have declining CD4+ T cells. The correlation between loss of this lymphocyte population and disease implicates CD4+ T cells in protection of the healthy individual from disease. CD4+ T cells have also been implicated in susceptibility in murine models of infection. While T-helper 1 type cell-mediated immunity (CMI) has been implicated in protection and recovery from both systemic and mucosal infection, it is also clear that mucosal and systemic immune response has elements of compartmentalization. This is emphasized by the susceptibility of the AIDS patient to oral mucosal but not systemic disease. The profile of T cells in the oral mucosa differs from that of peripheral blood. In recent years, T cells clones have been a useful tool to identify the specific antigens and their epitopes that stimulate the CMI response. However, the application of these protocols has been applied to lymphocytes of peripheral circulation and not those of the oral mucosa that are important in the response to oral pathogens. The goal of this proposal is to establish procedures for isolating CD4+ T cell clones that are derived from the T cell population of the oral mucosa in mice colonized with C. albicans. The successful development of this protocol has specific application that will provide a major tool for subsequent studies of host response and control of oral C. albicans. A successful protocol will also have more widespread application to study the mucosal response to components of the normal oral flora and agents of oral infection.