Engineering Acetobacter using Synthetic Biology to improve its industrial properties

细菌纤维素是一种超纯的碳水化合物聚合物，具有许多有用的性质。由于它的纯度，它具有高度的结晶结构和高的抗拉强度，此外还具有保水能力。因此，它引起了生物技术行业的极大兴趣，因为它提供了一种独特的材料，可以用于从滤膜到医疗器械的一系列应用。它主要是使用化学或酶处理来功能化的，然而我们使用合成生物学来使细菌纤维素功能化，以使用KTK Golden Gate工具包来设计Rhaeticus的代谢，Krhaeticus是醋酸菌家族的一种生物。在Khaeticus的生长过程中，它形成一层纤维素膜作为其生物膜。我们的目标是控制Rhaeticus的新陈代谢，以控制纤维素的生产。此外，我们希望将薄膜功能化，使其具有可在许多应用中使用的特性。我们还努力与Khaeticus和酵母建立群落，通过分泌纤维素结合结构域(CBDS)等蛋白质附着到纤维素上，同时保持初始蛋白质的能力，从而使薄膜具有功能性。不仅改变了纤维素膜的形态，而且还赋予了它反应功能。为其提供新的能力、抗拉强度和结晶度的纤维素工程是该项目的核心。

Bacterial cellulose is an ultra-pure carbohydrate polymer which has a myriad of useful properties. Due to its purity it has a highly crystalline structure and a high tensile strength, in addition to water holding potential. Therefore, it is of great interest to the biotechnology industry as it provides a unique material that can be used in a range of applications from filtration membranes to medical devices. It's is mainly functionalised using chemical or enzymatic processed however we set out to functionalise bacterial cellulose using Synthetic Biology to engineer the metabolism of K. rhaeticus, an organism from the Acetobacter family, using the KTK Golden Gate toolkit. During the growth of K. rhaeticus it forms a cellulose pellicle as its biofilm. We aim to engineer the metabolism of K. rhaeticus to control cellulose production. In addition, we wish to functionalise the pellicle giving it properties that can be used in many applications. We also endeavour to set up communities with K. rhaeticus and yeast to functionalise the pellicle by secreting proteins such as cellulose-binding-domains (CBDs) hybrid proteins to attach onto the cellulose while maintain the initial proteins abilities. Not only altering the morphology of the cellulose pellicle but also giving it a reactive function. The engineering of cellulose to provide it new abilities, tensile strength and crystallinity is the core of this project.