"Blood brain barrier on a Chip Model" for the study of drug delivery to the central nervous system

一个有用的血脑屏障体外模型应满足以下标准：溶质通透性的再现，限制性细胞旁通路的显示和生理上真实的结构，转运蛋白的功能表达，易于培养。脑内皮细胞、星形胶质细胞和周细胞的共培养导致血脑屏障转运蛋白的高表达和紧密连接，然而，最近的研究表明，将血流动力学纳入共培养可以更真实地表征血脑屏障。哈佛大学Wyss研究所在2016年发布的一份新闻稿中表示，他们已经制造出了人类血脑屏障“芯片上”的三维体外模型，该模型在相同的环境中自动定位，类似于在体内看到的排列

A useful BBB in vitro model should meet the following criteria: reproducibility of solute permeability, display of a restrictive paracellular pathway and physiologically realistic architecture, functional expression of transporters, and ease of culture. Co-cultures of brain endothelial, astrocytes and pericytes have led to high expression of BBB transporters and tight junctions, however, it has been shown recently that including hemodynamic flow into the co-cultures leads to a more realistic representation of the BBB. The Wyss Institute at Harvard University gave a press release in 2016 that they had created a 3-dimensional in vitro model of the human BBB "on-a-chip." which automatically oriented itself in a permutation similar to what is seen in vivo when put in the same environment