The development of an organ on a chip platform.

目的：设计和开发一种低成本的芯片平台器官，可以使用UltiMaker 2plus进行3D打印。这个平台必须使用电池供电，因为它考虑在第三世界国家使用可能经常发生停电。关键研究问题：如何密封外壳以确保70%的二氧化碳环境？由于尺寸限制，如何利用外壳内的空间以确保所有组件适合，因为目标是使用Ultiaker 2+3D打印机？研究各种方法/材料，以确保可以在显微镜下分析电池。将使用哪种编码平台来控制传感器和泵(Arduino或Raspberry派)？是否需要图形用户界面(图形用户界面)来显示外壳内的环境，或控制泵？将使用哪种动力系统使平台连续使用30天以上？简要方法：首先，必须概述设计要求和规范，以便构建用于指导设计过程的量化报表。随后，如摘要所述，将对照这些设计规范对当前的芯片上器官平台进行评估，以分析每个系统的有效性和局限性。概念设计将通过使用加权决策矩阵进行评估，以选择有效的设计进行制造。在制造之前，可以使用计算流体动力学(CFD)来可视化和研究流体流动(微流体流动)和施加在设计上的压力的影响。加热垫、外壳和芯片本身的材料选择将结合设计实施例进行。所考虑的材料必须能够承受热垫，价格实惠且透明。在用最有效的材料实现了最佳设计后，可以使用Ultiaker 2+3D打印机制造外壳，因为这台机器负担得起，而且在乌干达的伙伴大学可以买到。样机将在正确的细胞培养工作条件下进行测试，以评估芯片上器官平台相对于设计规范和目标的有效性。结合外壳设计，必须调查平台的其他因素，包括将使用哪些传感器(温度、湿度、PH值)？泵送机制。芯片将放置的热板。废物将被保存在哪里以供进一步测试。芯片设计和制造方法。由于3D打印的细胞可视化限制，这一点至关重要。细胞培养必须封闭在一个透明的封闭环境中，以便在显微镜下进行分析。一旦获得了完整的工作平台，就会对细胞培养支架进行进一步的调查。Metrigel和胶原支架可以被认为是生长乳腺癌细胞的支架。

Aim:To design and develop a low cost Organ On a Chip platform that can be 3D printed using an Ultimaker 2plus. This platform must be powered using batteries as it considers the use in 3rd world countries where power cuts may occur often.Key research questions: How to seal the enclosure to ensure a 70 per cent CO2 environment?How to utilize the space within the enclosure to ensure all components fit, due to the size constraint as the aim is to use an Ultimaker 2+ 3D printing machine?Investigate various methods/materials to ensure the cells can be analysed under a microscope.Which coding platform will be used to control the sensors and pump (Arduino or Raspberry pie)?Will a GUI (Graphical User Interface) be needed to display the ambient environment within the enclosure, or to control the pump?Which power system will be used to allow the platform to last for more than 30 consecutive days?Brief methodology:Initially, design requirements and specifications must be outlined in order to construct quantitative statements to guide the design process. Subsequently, as stated in the abstract, the current Organ on a Chip platforms will be evaluated against these design specifications to analyse the effectiveness and limitations of each system. Conceptual designs will be assessed by using a weighted decision matrix to select an effective design to fabricate. Prior to fabrication, Computational Fluid Dynamics (CFD) can be used to visualise and investigate the effects of the fluid flow (microfluidics flow), and the pressure applied on the design. The material selection of the heating pad, enclosure and the chip itself will be conducted in conjunction with the design embodiment. The material considered must be able to withstand the heat pad, affordable and transparent. Having achieved the optimal design with the most effective material, the enclosure can be fabricated using the Ultimaker 2+ 3D printing machine, as this machine is affordable and available at the Partnership University in Uganda. The prototype will be tested using the correct working conditions of cell culture in order to appraise the effectiveness of the Organ on a Chip platform against the design specifications and objectives.In conjunction with the enclosure design other factors of the platform must be investigated, these include; Which sensors will be used (Temperature, humidity, PH levels)?The pumping mechanism.The hot plate that the chip will sit on.Where the waste will be kept for further testing.The chip design and the fabrication method. This is crucial due to the cell visualization limitations that 3D printing has. The cell culture must be enclosed in a clear enclosure so it can be analsysed under a microscope.Once a full working platform has been achieved, further investigations regarding the cell culture scaffolding will be conducted. Metrigel and Collagen scaffolds could be considered to grow breast cancer cells.