CORE--IMMUNOLOGY AND MOLECULAR BIOLOGY

免疫学和分子生物学核心将提供免疫学和 支持该计划科学议程的病毒载量评估 项目。对于生物行为干预研究，项目0002和 0004，病毒载量的CD4T细胞计数将在 基线和认知行为压力管理(CBSM)后， 正如这些研究的实验设计中所定义的那样。项目0002 研究同性恋和双性恋男性；项目0004主要研究非洲人- 美国女性。对于项目0005，从项目0002和 0004将接受实验室反应性研究，包括前后 干预。以下免疫学检测将是该系列中的一种 采集受试者样本；淋巴细胞增殖分析(LPA) 对有丝分裂原植物血凝素(PHA)的反应；抗Epstein抗体 巴尔病毒(EBV)、人类疱疹病毒6型(HHV6)和单纯疱疹病毒 2型病毒(HSV2)自然杀伤细胞杀伤(NKCC)效应 肿瘤细胞靶点K562；四色流式细胞仪检测淋巴细胞表型 流式细胞术，测定CD4、CD8及其亚群的数量和百分比 这些，使用针对血统、激活、 分化和黏附分子。在这些样本上的细胞溶解 基于细胞内抗穿孔素抗体的NK细胞潜能 将对流式细胞术进行评估并确定其程度 L-选择素(CD62L)、LFA-1(CD11a)和CD56的荧光表达 强度测量。0005项目还将包括体外培养 儿茶酚胺和细胞因子对NK细胞影响的实验研究 活动，这将由这个核心来完成。

The IMMUNOLOGY AND MOLECULAR BIOLOGY CORE will provide the immunology and viral load assessments in support of the scientific agenda of this Program Project. For the biobehavioral intervention studies, Projects 0002 and 0004, viral load measurements of CD4 T cell counts will be made at baseline and just after a cognitive behavioral stress management (CBSM), as defined in the experimental design for these studies. Project 0002 studies gay and bisexual men; Project 0004 studies predominantly African- American women. For Project 0005 selected subjects from Projects 0002 and 0004 will undergo a laboratory reactivity study both pre-and post- intervention. The following immunology assays will be one on the serial samples collected on subjects; lymphocyte proliferation assays (LPA) in response to the mitogen phytohemagglutinin (PHA); antibodies to Epstein Barr virus (EBV), human herpes virus type 6 (HHV6) and herpes simplex virus type 2 (HSV2) natural killer cell cytotoxicity (NKCC) against the tumor cell target, K562; lymphocyte phenotypes using 4-color flow cytometry, determining number and percent of CD4, CD8 and subsets of these, using monoclonal antibodies specific for lineage, activation, differentiation and adhesion molecules. On these samples cytolytic potential of NK cells using anti-perforin antibody-based intracellular flow cytometry will be assessed and determination made of degree of expression of L-selectin (CD62L), LFA-1 (CD11a) and CD56 by fluorescence intensity measurements. Project 0005 will also include in vitro experiments to measure the effects of catecholamines and cytokines of NK activity, that will be done by this core.