POTASSIUM CHANNEL ACTIVATION BY VOLATILE ANESTHETICS

描述：(改编自申请者的摘要)这是第一个奖项 建议通过以下方式检查基线K+泄漏通道的激活 挥发性麻醉剂。具体目标是(1)确定其作用机制 通过检查一系列挥发性麻醉剂如何激活通道 浓度改变了通道的属性，除了研究基本的 该经络类型的生理学和药理学；(2)，鉴定和 描述挥发性麻醉剂起作用的结合部位 检测它们阻断内源性信使活动的能力 作为花生四烯酸和卤代非麻醉药的作用能力 并抑制异氟烷的作用，以及(3) 确定这些通道在调节神经元兴奋性中的作用 和突触传递。研究将在小脑进行 全细胞破损贴片和切除贴片对颗粒神经元的影响 培养和薄片培养神经元的技术。

DESCRIPTION: (Adapted from the applicant's abstract) This FIRST award proposal is to examine the activation of baseline K+ leak channels by volatile anesthetics. Specific aims are (1) to determine the mechanism of channel activation by examining how volatile anesthetics in a range of concentrations alter channel properties, in addition to study the basic physiology and pharmacology of this channel type; (2), to identify and characterize the binding sites at which volatile anesthetics act by examining their ability to occlude actions of endogenous messengers such as arachidonic acid and the ability of halogenated non-anesthetics to act on the channels and to inhibit the actions of isoflurane, and (3) to determine the role of these channels in modulating neuronal excitability and synaptic transmission. Studies will be carried out in cerebellar granule neurons using whole cell ruptured patch and excised patch techniques on neurons in culture and in thin slices.