PREVALENCE OF HEPATITIS G VIRUS

GB型肝炎病毒C/G型肝炎病毒（统称为HGV） 是一种最近发现的RNA病毒，似乎是通过 胃肠外暴露于受污染的血液和血液制品， 与人类临床肝炎有关。 本研究的目的 目的评价HGV在HCV感染者中的流行情况， 对HCV感染严重程度的影响， 血清丙氨酸氨基转移酶（ALT）。 HGV的患病率为 还在HCV污染的免疫球蛋白静脉注射产品中进行了研究 （IGIV）在引入病毒灭活之前生产 处理和接收这些批次。 巢式引物，特异性 针对HGV的5 '端非编码区，设计了一系列特异性引物， 100例慢性HCV感染者，10例HCV RNA阳性IGIV 这些产品的接收者中有36人。 HGV特异性 PCR产物通过对一些扩增的DNA序列进行测序来确认。 并将结果与已发表的原型序列进行比较 HGV RNA 100例HCV感染者中23例（23%）检出HGV RNA 患者 HCV-HGV合并感染者ALT水平低于HCV-HGV合并感染者， 只有HCV感染者。 检测的10个IGIV批次中的2个（20%） HGV RNA阳性，然而，没有来自受体的血清样品 IGIV中有HGV RNA。 这些结果表明HGV感染经常与HCV一起发生 感染，可能是因为类似的传播模式和风险 因素 然而，HCV感染的严重程度不受 HGV的存在，如ALT水平所示。 这项研究还表明， IGIV产品中的HGV-RNA传播给接受者 似乎不如HCV有效。

Hepatitis GB virus C/hepatitis G Virus (collectively will be called HGV) is a recently identified RNA virus which appears to be transmitted by parenteral exposure to contaminated blood and blood products, and may be associated with clinical hepatitis in humans. The objective of this study was to evaluate the prevalence of HGV in HCV infected individuals, and its effect on the severity of HCV infection as determined by the level of alanine aminotransferase (ALT) in the serum. The prevelance of HGV was also investigated in HCV contaminated Immune Globulin Intravenous products (IGIV) manufactured prior to the introduction of viral inactivation processing and in recipients of these lots. Nested primers, specific for the 5' noncoding region of HGV, were designed and used to test serum samples from 100 chronic HCV infected patients, 10 HCV RNA positive IGIV lots and 36 of the recipients of these products. HGV specificity of the PCR products was confirmed by sequencing a number of the amplified products and comparing the results with the published prototype sequence for HGV RNA. HGV RNA was detected in 23 of the 100 (23%) HCV infected patients. The level of ALT was lower in HCV-HGV coinfected patients than those with HCV infection alone. Two of the 10 (20%) IGIV lots tested positive for HGV RNA, however, none of the serum samples from recipients of IGIV contained detectable HGV RNA. These results indicate that HGV infection occurs frequently with HCV infection, probably because of similar modes of transmission and risk factors. However, the severity of HCV infections is not effected by the presence of HGV, as indicated by the level of ALT. This study also shows that the transmission of HGV-RNA from IGIV product to the recipients appear to be less efficient than that seen for HCV.