Quantitative Detection of Cytomegalovirus by Using a Commercially Developed Resea

巨细胞病毒（CMV）可以是毁灭性的， 接受过同种异体骨髓移植的患者。 快速检测这些患者的CMV感染， 挽救生命，允许及时给予抗CMV药物。 临床上也需要定量测量CMV 存在于临床样本中，以允许监测治疗， 预测抗药性变异的出现。研究检测 由商业供应商开发，允许使用 定量聚合酶链反应（PCR）检测CMV 从全血中提取的DNA我们将评估该PCR检测 用美国人收集的外周血白细胞， 医学实验室，并与CMV的结果进行比较 抗原血症定量测定（在American Medical 早期检测CMV pp65的存在 多形核白细胞和单核细胞中的结构蛋白。

Cytomegalovirus (CMV) can be devastating in the patient who has undergone an allogeneic bone marrow transplant. Rapid detection of CMV infection in these patients can be life-saving, allowing the prompt administration of anti-CMV drugs. There is also a clinical need for a quantitative measure of the CMV present in the clinical specimen to allow for monitoring therapy and predicting emergence of drug-resistant variants. A research assay has been developed by a commercial vendor allowing the use of quantitative polymerase chain reaction (PCR) to measure the CMV DNA extracted from whole blood. We will evaluate this PCR assay by using peripheral blood leukocytes collected by American Medical Laboratories and compare the results with the CMV antigenemia quantitative assay (performed at American Medical Laboratories) that detects the presence of CMV pp65 early structural protein in polymorphonuclear leukocytes and monocytes.