Characterization of CTIP1 in Immune Cells

描述(申请人提供)：造血和细胞免疫 由生长因子、细胞相互作用和 调控的基因表达。我们最近发现了一种新的转录 免疫系统中表达的因子和间接证据 提示该蛋白在免疫系统的发育和功能中起作用。 细胞。COUP Tf相互作用蛋白1(CTIP1)是一个新的转录因子家族成员。 C2H2锌指蛋白，最近被证明具有 基因转录的调节剂。CTIP1通过物理途径调节转录 与鸡卵清蛋白上游启动子转录因子(COUP)相互作用 Tf)孤儿核受体，或通过直接与共识CTIP反应结合 元素。CTIP1在小鼠骨髓、脾、淋巴结、 纯化的B细胞和树突状细胞。这项研究的目标是测试 CTIP1在免疫发育和功能中的作用假说 细胞。拟议研究的目的是：(1)描述 CTIP1在发育中免疫细胞中的表达：(2)阐明可能的机制 CTIP1在白细胞中的功能：和(3)确定靶基因 由CTIP1调控。确定这种蛋白质的功能是非常重要的 生物医学的兴趣不仅是因为它是真正的转录 这不仅是因为这个基因最近被鉴定为 逆转录病毒插入导致细胞转化和 白血病的发生。在成功完成这项建议后，我们的 预计这些研究将促进我们对造血的了解。 和免疫细胞调节，并进一步阐明分子和细胞 CTIP1的功能。

DESCRIPTION (provided by applicant): Hematopoiesis and cellular immunity are complex processes controlled by growth factors, cellular interactions, and regulated gene expression. We have recently identified a novel transcription factor which is expressed in the immune system and circumstantial evidence suggests a role for this protein in the development and function of immune cells. COUP TF-Interacting Protein 1 (CTIP1) is a member of a novel family of C2H2 zinc finger proteins that have recently been demonstrated to function as modulators of gene transcription. CTIP1 regulates transcription by physically interacting with Chicken Ovalbumin Upstream Promoter Transcription Factor (COUP TF) orphan nuclear receptors, or by directly binding to consensus CTIP response elements. CTIP1 is highly expressed in murine bone marrow, spleen, lymph nodes, and purified B-cells and dendritic cells. The goal of this study is to test the hypothesis that CTIP1 plays a role in the development and function of immune cells. The aims of the proposed research are: (1) to characterize the expression of CTIP1 in developing immune cells: (2) to elucidate possible functions of CTIP1 in leukocytes: and (3) to identify target genes that are regulated by CTIP1. Defining the function of this protein is of great biomedical interest not only because of its role as a bona fide transcription factor but also because this gene was recently identified as a site of retroviral insertion that results in cellular transformation and leukemogenesis. Upon the successful completion of this proposal, our expectation is that these studies will advance our knowledge of hematopoiesis and immune cell regulation and further elucidate the molecular and cellular functions of CTIP1.