Whole Animal ELISA for Liver Toxicity

描述（由申请人提供）：斑马鱼具有与人类相似的组织和器官发育的形态学和分子基础，是一种理想的脊椎动物毒性试验模型。与小鼠和大鼠相比，斑马鱼的繁殖成本不高，胚胎发生速度快，而且由于它们的胚胎是透明的，所以易于分析。此外，药物传递简单，对毒性有良好的剂量反应性。目前，斑马鱼毒性试验采用致死率、胚胎存活率、行为和器官畸形的显微检查作为评估毒性的一般参数。然而，对于大规模的毒性筛选，定性目视检查是不合适的。利用斑马鱼胚胎，这个I期SBIR旨在开发一种基于微孔板的酶联免疫吸附试验来量化肝毒性。一种快速、定量的筛选发育器官毒性化合物的方法将有助于进一步药物开发的先导化合物的选择。

DESCRIPTION (provided by applicant):The zebrafish, which has a morphological and molecular basis of tissue and organ development similar to that found in humans, is an ideal, vertebrate animal model for toxicity testing. In contrast to mice and rats, zebrafish are inexpensive to breed, undergo rapid embryogenesis and are easy to analyze because their embryos are transparent. Furthermore, drug delivery is simple and good dose responsiveness to toxicity has been observed. Currently zebrafish toxicity assays use lethality, embryo survival rate, behavior and microscopic examination of organ malformation as general parameters for assessing toxicity. However, for large-scale toxicity screening, qualitative visual inspection is not suitable. Using zebrafish embryos, this Phase I SBIR aims to develop a microplate-based ELISA assay to quantify liver toxicity. A rapid, quantitative assay for screening compounds for developmental organ toxicity will facilitate selection of lead compounds for further drug development. PROPOSED COMMERCIAL APPLICATIONS: By providing a rapid, quantitative, and inexpensive method for organ toxicity testing, the zebrafish assay will facilitate drug screening and toxicity testing in the pharmaceutical and chemical industries.