Sensitive, Integrating Multi-Waveguide Biosensor

生物传感器通过检测蛋白质、酶、细菌、毒素、药物、污染物、DNA等，具有广泛的适用性，例如医疗诊断、传染病、环境传感和食品污染。更好的生物传感器的开发将产生深远的社会影响，改善医疗保健和生活质量。我们建议开发一种集成的多波导生物传感器，其中发光物种在传感表面上的照明不是基于倏逝波。该生物传感器能够：- 测定下限-高灵敏度-高选择性-同时分析多种分析物-波导之间的低干扰，以及-增强背景和发射信号之间的区分。初步实验已经证明了其中的一些特征。该研究将优化，制造和测试这种新型的生物传感器。第一阶段研究将：开发一种方法来分析波导表面上的荧光，并在光线跟踪代码中实现该方法，应用该方法来优化波导设计，制造最佳波导设计，用分析物对波导进行实验测试，并将实验数据与参考实验数据和模拟结果进行比较。拟议的商业应用：生物传感器可应用于医疗诊断、传染病、环境传感和食品污染，检测蛋白质、酶、细菌、抗体、毒素、药物、污染物等，市场每年超过100亿美元。

Biosensors have wide applicability, such as medical diagnostics, infectious diseases, environmental sensing and food contamination, by detecting proteins, enzymes, bacteria, toxins, drugs, pollutants, DNA, etc. The development of better biosensors will have far-reaching societal impact, improving both health care and quality of life. We propose to develop an integrating multi-waveguide biosensor, where the illumination of the luminescent species on the sensing surface is not based on evanescent waves. The biosensor is capable of: - Low limits of determination - High sensitivity - High selectivity - Simultaneous analysis of multiple analytes - Low interference between waveguides, and - Enhanced discrimination between background and emitted signal. Preliminary experiments have demonstrated some of these features. The proposed research will optimize, fabricate and test this new type of biosensor. The Phase I research will: develop a method to analyze fluorescence on waveguide surfaces and implement it in a ray tracing code, applying the method to optimize waveguide designs, fabricate the best waveguide designs, experimentally test the waveguides with analyte, and compare the experimental data to reference experimental data and simulation results. PROPOSED COMMERCIAL APPLICATIONS: Biosensors can be applied to medical diagnostics, infectious diseases, environmental sensing and food contamination, to detect proteins, enzymes, bacteria, antibodies, toxins, drugs, pollutants, etc. The market is over $10 billion per year.