African Trypanosome Genome Sequencing

描述（申请人摘要）：非洲锥虫是一种原生动物 这种寄生虫会导致一种叫做昏睡病的致命疾病， ngana在国内牲畜。它也是一种很有价值的实验生物， 他的研究为生物学的重大发现做出了贡献。测序 布氏锥虫参考株TREU 927/4 GUTat 10的核基因组。1 1998年，基因组研究所（NIAID U 01） AI 43062）和桑格中心（惠康信托）。自那以后，TIGR提供了 染色体图谱的宝贵支架， BAC和P1末端序列，以及大约37，000个基因的鸟枪序列。 的发现1999年，我们开始了第二、第四和第六号染色体的测序， 并将于短期内制作第一个完整的 非洲锥虫巨大染色体（chr II）的序列。桑格 中心进行了百万例染色体全染色体测序 鸟枪和脱脂测序的映射基因组克隆。他们希望完成 我很快就完成了chr I的测序，并获得了对这三个基因进行测序的资金。 T.布氏杆菌（IX、X和Xi）。剩余染色体 (III第五、第七和第八条）。 我们现在建议（i）确定剩余四个的7 Mb序列 使用成功用于测序chr II的相同方法， 将现有的基因组资源与基于 使用来自BAC克隆的末端序列来选择用于测序的最佳克隆; (ii)分析和注释该序列;以及（iii）制作该序列， 分析数据提供给研究界。全基因组序列 中医布鲁塞将在许多层面提供宝贵的信息和好处， 包括（1）鉴定参与细胞基本功能的基因， 真核细胞，（2）容易，廉价和快速克隆基因编码 蛋白质在世界各地的实验室正在积极研究，（3）立即 获得基因及其产物用于功能/结构研究，（4） 基于候选基因的代谢途径预测，以及（5） 通过与其它基因产物比较来鉴定寄生虫特异性基因产物 基因组

DESCRIPTION (Applicant's Abstract): The African trypanosome is a protozoan parasite that causes a fatal disease called sleeping sickness in humans and ngana in domestic livestock. It is also a highly valued experimental organism, whose study has contributed fundamental discoveries in biology. Sequencing of the nuclear genome of Trypanosoma brucei reference strain TREU927/4 GUTat10. 1 commenced in 1998 at both The Institute for Genomic Research (NIAID U01 AI43062) and Sanger Centre (Wellcome Trust). TIGR has since provided an invaluable scaffold for chromosome mapping in the form of approximately 11,000 BAC and P1 end sequences, and approximately 37,000 shotgun sequences for gene discovery. In 1999, we initiated the sequencing of chromosomes II, IV and VI, using a 'map-as-you-go' approach; and will shortly produce the first complete sequence of an African trypanosome megabase chromosome (chr II). The Sanger Centre has carried out megabase chromosome sequencing by whole chromosome shotgun and skim sequencing of mapped genomic clones. They expect to complete the sequence of chr I very soon and have secured funding to sequence the three largest chromosomes of T. brucei (IX, X and XI). The remaining chromosomes (III, V, VII and VIII) were provisionally adopted by TIGR. We now propose to (i) determine the 7 Mb sequence of the four remaining chromosomes using the same approach used successfully to sequence chr II, by combining existing genome resources with a map-as-you-go strategy based on the use of end sequences from BAC clones to select optimal clones for sequencing; (ii) analyze and annotate the sequence; and (iii) make the sequence and analysis data available to the research community. The complete genome sequence of T. brucei will provide invaluable information and benefits at many levels, including (1) identification of genes involved in basic functions of the eukaryotic cell, (2) easy, inexpensive and fast cloning of genes encoding proteins being actively studied in laboratories around the world, (3) immediate access to genes and their products for functional/structural studies, (4) prediction of metabolic pathways on the basis of candidate genes, and (5) identification of parasite-specific gene products by comparison with other genomes.