SEQUENCING 12 MB OF THE SCHISTOSOMA MANSONI GENOME

对 12 MB 曼索尼血吸虫基因组进行测序

• 批准号: 6511416
• 负责人: Najib M El-Sayed
• 金额: $ 351.79万
• 依托单位: INSTITUTE FOR GENOMIC RESEARCH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2006-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6511416
• 关键词: Internet; Schistosoma mansoni; artificial chromosomes; computer assisted sequence analysis; fluorescent in situ hybridization; genetic library; genetic mapping; genome; nucleic acid probes; nucleic acid sequence; restriction fragment length polymorphism

DESCRIPTION (Investigator's Abstract): Schistosoma mansoni is a trematode parasite that causes schistosomiasis. After malaria, schistosomiasis is the most prevalent tropical disease and leading cause of severe morbidity in developing nations. The disease is endemic in 74 developing countries, with estimates of 600 million people at risk and over 200 million infected. The schistosome genome is approximately 270 Mb, organized into 8 chromosome pairs. Under the auspices of the WHO, an international consortium was established in the 90's to encourage and coordinate genomic research on schistosomes. Its overall aim is to gain knowledge about the genome of these parasites in order to further understanding of their biology, mechanisms of drug resistance and antigenic variation that determine escape from the host's immune system. One of the main objectives of this program is the discovery and characterization of new genes of S. mansoni and in an attempt to search for new targets for drugs and vaccine development. As a preliminary step to achieving this goal, Dr. Cummings and her colleagues at TIGR will sequence 7 Mb of discontinuous DNA sequence using a BAC-end sequencing approach to obtain STS randomly distributed across the genome at about every 20 kb. Flourescent in situ hybridization (FISH) to mitotic metaphase chromosomes will be used to map the chromosomal locations of hundreds of these markers. The PI will also oversee sequencing and annotation of 5 Mb on contiguous DNA sequence from chromosome 3, ch3. A minimum BAC tiling path for a region near the centromere and long arm of ch3 is currently being constructed. For this purpose, terminal sequences and restriction patterns will be defined for each BAC clone, A series of 10 percent overlapping BACs will be selected and used to prepare BAC shotgun small insert sublibraries which will be sequenced to 8 fold coverage. The DNA sequence from this region will be assembled and following gap closure will be full annotated leading to the identification of perhaps hundreds of new schistosome genes which will revel new insights into schistosome chromosome and genome organization.

描述（研究者摘要）：曼氏血吸虫是一种吸虫

项目成果

Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni.

Mansoni血吸虫中性接触引起的基因表达的微阵列分析。

• DOI: 10.1186/1471-2164-8-181
• 发表时间: 2007-06-20
• 期刊: BMC GENOMICS
• 影响因子: 4.4
• 作者: Waisberg, Michael;Lobo, Francisco P.;Cerqueira, Gustavo C.;Passos, Liana K. J.;Carvalho, Omar S.;Franco, Gloria R.;El-Sayed, Najib M.
• 通讯作者: El-Sayed, Najib M.

Identification of Schistosoma mansoni microRNAs.

• DOI: 10.1186/1471-2164-12-47
• 发表时间: 2011-01-19
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Simões MC;Lee J;Djikeng A;Cerqueira GC;Zerlotini A;da Silva-Pereira RA;Dalby AR;LoVerde P;El-Sayed NM;Oliveira G
• 通讯作者: Oliveira G

Evolution of sex chromosomes ZW of Schistosoma mansoni inferred from chromosome paint and BAC mapping analyses

• DOI: 10.1016/j.parint.2012.07.007
• 发表时间: 2012-12-01
• 期刊: PARASITOLOGY INTERNATIONAL
• 影响因子: 1.9
• 作者: Hirai, Hirohisa;Hirai, Yuriko;LoVerde, Philip T.
• 通讯作者: LoVerde, Philip T.

Identification of immediate response genes dominantly expressed in juvenile resistant and susceptible Biomphalaria glabrata snails upon exposure to Schistosoma mansoni.

• DOI: 10.1016/j.molbiopara.2009.09.009
• 发表时间: 2010-01
• 期刊: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
• 影响因子: 1.5
• 作者: Ittiprasert, Wannaporn;Miller, Andre;Myers, Jocelyn;Nene, Vish;El-Sayed, Najib M.;Knight, Matty
• 通讯作者: Knight, Matty

Schistosoma mansoni: Microarray analysis of gene expression induced by host sex.

• DOI: 10.1016/j.exppara.2008.09.005
• 发表时间: 2008-12
• 期刊: Experimental parasitology
• 影响因子: 2.1
• 作者: Michael Waisberg;Francisco Pereira Lobo;Gustavo C. Cerqueira;L. K. Passos;Omar dos Santos Carvalho;N. El-Sayed;G. R. Franco