CORE--HISTOLOGY AND IMMUNOHISTOCHEMISTRY

在加州大学圣地亚哥分校，许多研究人员正在进行研究，需要对人类和小鼠肿瘤及其同源正常组织进行显微镜分析。其他人正在研究通过遗传和表观遗传干预在胚胎和成年动物中引起的变化的细节，以了解调节正常和肿瘤发育的机制。这些正在研究与癌症相关主题的研究人员需要随时获得负担得起的高质量组织学和免疫组织化学技术服务以及专家组织病理学建议。该资源已经为中心成员提供了至关重要的服务，随着人们越来越广泛地认识到组织的显微镜评价在癌症研究中的重要性，其重要性将不断增加。该核心服务在生化或分子生物学分析开始之前对活检样本中肿瘤组织的存在进行重要的质量控制。还证实了在被视为此类实验的正常对照的组织中不存在肿瘤。此外，通过利用这一核心设施的服务，从胚胎阶段到成年，对遗传改变小鼠的组织进行完整的组织学（表型）分析成为可能。利用这些服务还可以对人类癌症的众多小鼠模型进行详细分析。组织学共享资源为所有同行评审的癌症中心成员提供高质量的组织学和免疫组织化学服务。它还与其他UCSD NCI资助的癌症中心共享资源密切联系，特别是分子病理学，转基因小鼠和数字成像，以创建一个互动的共享资源组，用于研究来自人类病理标本的组织样本，来自人类病理标本，携带人类异种移植的实验动物和转基因动物（转基因或“敲除”小鼠）。

At UCSD many investigators are now conducting research which requires the microscopical analysis of human and murine tumors and their cognate normal tissues. Others are examining the details of changes induced in embryonic and adult animals by genetic and epigenetic intervention, so as to understand the mechanisms regulating normal and neoplastic development. These investigators who are studying topics relevant to cancer need ready, affordable access to high quality histological and immunohistochemical technical services and to expert histopathological advice. This resource already provides pivotally important services to the Members of the Center and its importance will grow as it becomes more widely appreciated that microscopic evaluation of tissue is critical in cancer research.. This core services provides vital quality control of the presence of tumor tissue in a biopsy sample before biochemical or molecular biological analysis begins. Establishing the absence of tumor in tissue that is regarded as a normal control for such experiments is also confirmed. In addition, by utilizing the services of this core facility, a complete histologic (phenotypic) analysis of tissues from genetically altered mice, is made possible, from the embryo stage to the adult. Utilization of the services also allows a detailed analysis of the numerous mouse models of human cancer. The Histology Shared Resources provides high quality histology and immunohistochemistry services to all peer-reviewed Cancer Center members. It also liaises closely with other UCSD NCI-funded Cancer Center Shared Resources, especially Molecular Pathology, Transgenic Mouse and Digital Imaging, to create an interactive group of shared resources for research of tissue samples from human pathological specimens, from human pathological specimens, experimental animals carrying human xenotransplants, and genetically altered animals (Transgenic or "knock-out" mice).