CORE--FLOW CYTOMETRY AND CELL SORTING

流式细胞术和细胞分选设备由癌症中心于1980年建立。自1981年以来，它一直得到NCI核心支持补助金的批准和资助。Jonni Moore博士自1990年以来一直指导流式细胞术和细胞分选设施。他拥有免疫学博士学位和超过15年的流式细胞术经验，并担任流式细胞术设备制造商（即Becton Dickinson）和其他流式细胞术实验室（例如SmithKline Beecham， Merck）的专家顾问。Charles Pletcher， MCI，技术总监，自1987年以来一直在该设施工作。在当前项目期间，该设施发展迅速。这是因为在取得新技术和征聘专家工作人员期间，业务有了重大改进。在本项目期间购买的设备（facadvantage和FACSCalibur），它现在拥有与最先进的流式细胞仪设备相当的能力。在当前项目期间，核心的总体使用量增加了67%。获得同行评审资助的癌症中心成员的使用率为77%，所有癌症中心成员的使用率为总设施使用率的86%。

The Flow Cytometry and Cell Sorting Facility was established by the Cancer Center in 1980. It has been continuously approved and funded by the NCI Core Support Grant since 1981. Jonni Moore, PhD, has directed the Flow Cytometry and Cell Sorting Facility since 1990. Dr. Moore has a doctorate in immunology and over fifteen years of flow cytometry experience, and serves as an expert consultant to manufacturers of flow cytometry equipment (i.e., Becton Dickinson) and to other flow cytometry laboratories (e.g., SmithKline Beecham, Merck). Charles Pletcher, MCI, Technical Director has been with the Facility since 1987. The Facility has grown tremendously during the current project period. This has resulted from major operational improvements, during acquisition of newer technology, and recruitment of expert staff. With the equipment purchased during the current project period (FACSVantage and FACSCalibur), it now possesses capabilities equal to those of the most sophisticated flow cytometry facilities. Overall usage of the core has increased 67% during the current project period. Usage by Cancer Center members with peer-reviewed funding is 77% and usage by all Cancer Center members is 86% of total facility usage.