CORE--CELL BIOLOGY

细胞生物学核心将与每个项目互动并为其提供支持 在司令部。中心目标将是提供具有良好特征的初级 用作模型和用于细胞生物学研究的人类细胞和细胞系 与急性呼吸窘迫综合征的肺损伤有关 (ARDS)，就像我们在当前资金期所做的那样。此外，就像在 当前资金期限。主要的细胞类型将包括新分离的 人中性粒细胞、单核细胞和其他白细胞及原代培养 内皮细胞。此外，我们还将发展、特色化和 为项目提供各种专门研究的细胞系， 包括在核心培养的一种肺内皮细胞系。我们 关注人类细胞类型，因为有直接的相关性，但也会 提供实验动物的培养细胞或已建立的细胞系 在有用的时候。核心人员还为SCOR调查人员提供细胞生物学方面的建议 协议，帮助确定和开发新的准备工作，试验和开发 专门的技术、分析和模型，在某些情况下执行 使用SCOR或用于SCOR的专门细胞生物学研究或分析 调查人员。核心将促进和扩大基本调查 在SCOR中，这将导致对生物特征的新认识 急性呼吸窘迫综合征的肺损伤。

The Cell Biology Core will interact with and support each of the projects in the SCOR. The central aim will be to provide well-characterize primary human cells and cell lines for use as models and in cell biologic studies that are relevant to lung injury in acute respiratory distress syndrome (ARDS), as we have done in the current funding period. Also, as in the current funding period. the major cell types will include freshly isolated neutrophils, monocytes, and other leukocytes and primary cultures of human endothelial cells. In addition, we will also grow, characterize, and furnish to the projects a variety of cell lines for specialized studies, including a pulmonary endothelial cell line developed in the Core. We focus on human cell types, because of immediate relevance, but also will provide cultured cells or established cell lines from experimental animals when useful. Core personnel also advise SCOR investigators on cell biology protocols, help identify and develop new preparations, pilot and develop specialized techniques, assays, and models, and in some cases perform specialized cell biologic studies or assays with or for SCOR investigators. The core will foster and extend fundamental investigations in the SCOR that will lead to new knowledge of the biologic features of lung injury in ARDS.