Core--Genetic analysis

描述（摘自应用程序） 西奈山超级基金基础研究计划遗传分析核心，核心 E，将进行生物医学所需的所有基于核酸的测定， 研究项目10和3。内核会提取并储存人类DNA编码 用于基因型分析的样本。将通过PCR-RFLP确定双等位基因基因型 （聚合酶链反应-限制性片段长度多态性）或 等位基因特异性PCR。竞争性技术可以替代成本 还原这个核心的工作将决定和描述人类 已知影响铅代谢的多态性[ALAD、VDR和HFE]。还 核心将研究影响有机氯代谢的多态性， 可能有助于基因-环境相互作用， 帕金森病的发生[CYP 2D 6，CYP 2 E1，PON 1和GSTP 1，以及 PON 2和PON 3中已知和新衍生的多态性]。额外 将根据研究项目的要求确定多态性 董事们。核心将确定小鼠WNT 4、WNT 5a和WNT 7a基因型， 基因敲除小鼠的杂交后代，以支持研究项目9。的 核心将寻找人类WNT多态性的三个小鼠WNT基因 在项目9中学习。还将研究人PON 2和PON 3。核心 将根据董事的要求寻找其他人类多态性 的研究项目。

DESCRIPTION (Taken from application) The Mount Sinai Superfund Basic Research Program Genetic Analysis Core, Core E, will carry out all nucleic acid based assays required for the biomedical Research Projects 10 and 3. The Core will extract and store coded human DNA samples for genotype analyses. Biallelic genotypes will be determined by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) or allele-specific PCR. Competing technologies may be substituted for cost reduction. The work of this Core will determine and characterize human polymorphisms known to affect lead metabolism [ALAD, VDR and HFE]. Also, the Core will examine polymorphisms affecting metabolism of organochlorines that may contribute gene-environment interactions potentially relevant to the genesis of Parkinson's Disease [CYP2D6, CYP2E1, PON1 and GSTP1, as well as known and newly-derived polymorphisms in PON2 and PON3]. Additional polymorphisms will be determined as requested by the Research Project Directors. The Core will determine mouse WNT4, WNT5a and WNT7a genotypes of progeny in crosses of knock-out mice in support of Research Project 9. The Core will search for human WNT polymorphisms for the three mouse WNT genes studied in Project 9. Human PON2 and PON3 will also be investigated. The Core will search for additional human polymorphisms as requested by the Directors of the Research Projects.