CORE--BIOCHEMICAL AND MOLECULAR

生化核心实验室的这个计划项目赠款将提供一个 高度专业化、装备精良和专业化的综合工作人员 能够进行各种放射免疫测定、生化 以及项目研究人员要求的分子程序。没有单一 仅使用R 01支持的研究者实验室将 能够装备和配备这种分析资源或人员 他们将用放射化学方法分析样本中P450和NOS的活性， HPLC法测定脑脊液微透析中20-HETE和雌二醇的有效性 使用新的荧光HPLC分析。该核心还将测量IP 3，DAG， 小胶质细胞和雌胶质细胞产物在脑和脑血管系统中的作用。 此外，我们将使用免疫印迹法测量P4504和NOS蛋白， 用竞争性RCPR进行分子测量。访问 中心设施的分析专业知识和设备至关重要 每一个拟议的项目，并证明了大量的 所需的生化和分子测定 问题研究

Biochemical core lab of this program project grant will provide a consolidated highly specialized well-equipped and professional staff unit capable of performing a variety of radio-immunoassay, biochemical and molecular procedures as required by project investigators. No single investigator laboratory supported with R01 support alone would be capable of equipping and staffing this analytical resource or personnel who will analyze samples for P450 and NOS activities using radiochemical HPLC assay measurements, 20-HETE and EETs in CSF microdialysis efficient using new fluorescent HPLC assays. This core will also measure IP3, DAG, microglial and estroglial products in the brain and cerebrovasculature. In addition, we will measure P4504 and NOS protein using immunoblot and measure molecular measurements with competitive RCPR. Access to the analytical expertise and equipment in the central facility is essential to each of the proposed projects and justified by the large number of biochemical and molecular determinations required for the proposed studies.