Vibrational Nanospectroscopy of Biosubstrate Surfaces and Molecular Films

该项目需要在与头发和个人护理产品相关的模型化学处理前后对各种生物/商业相关基质进行成像和光谱分析。这些包括处理过的和未处理过的头发和皮肤模型，都是真实的基底，如微切面头发和模型系统。要研究的模型系统包括但不限于主要由DPPC和DPPE组成的生物脂膜（包括单层和双层），这些生物脂膜系统将作为与个人护理领域和基础学术研究相关的模型脂膜系统进行研究。成像将在部门Nano-IR2仪器上进行，该仪器包括原子力显微镜和相关的中红外激光器（AFM-IR），用于亚衍射极限光谱和光谱测绘，并在共聚焦拉曼显微镜上进行额外的光谱测绘。测绘研究的目的是阐明不同表面化学物质在大尺度（约1微米）和纳米尺度（约40纳米）上的定位。薄膜的结构和构象顺序将通过使用和频产生光谱（SFG）和辅助使用偏振调制反射吸附光谱（PMIRRAS）和衰减全内反射光谱（ATR）来确定。ATR研究的进一步发展计划是通过使用极化调制ATR，以便在研究水合脂质双分子层系统时最小化液相的贡献。

The project entails the imaging and spectroscopy of a variety of biologically/commercially relevant substrates before and after treatment with model chemical treatments relevant to hair and personal care products. These are to include treated and untreated hair and skin models, both as real substrates such as microtomed hair cross sections and model systems. The model systems to be investigated comprise but will not be limited to biolipid membranes (both as monolayers and as bilayers) composed primarily of DPPC and DPPE, which will be investigated as model lipid membrane systems relevant to both the personal care field and as fundamental academic studies.Imaging will be carried out on the departmental Nano-IR2 instrument comprising of an atomic force microscope with associated mid IR laser (AFM-IR) for sub diffraction limited spectroscopy and spectral mapping with additional spectral mapping carried out on a confocal Raman microscope. Mapping studies aim to elucidate the localisation of different surface chemistries both on the large scale, circa 1 micron and also at the nanoscale at circa 40nm. The structure and conformational ordering of the films is to be determined through the use of Sum Frequency Generation spectroscopy (SFG) with the ancillary usage of polarization modulation reflection adsorption spectroscopy (PMIRRAS) and attenuated total internal reflectance (ATR) spectroscopy. Further development of the ATR investigation is planned via the use of polarization modulated ATR in order to minimise the contribution from the liquid phase when studying hydrated lipid bilayer systems.