A study on microplastics interference in mammalian cells metabolism via Infrared Microimaging and Nanospectroscopy

通过红外显微成像和纳米光谱研究微塑料干扰哺乳动物细胞代谢

• 批准号: 2868550
• 金额: --
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2868550
• 关键词: study; microplastics; interference; mammalian; cells

The aim is to develop an experimental approach combining live cell Syncrotron Radiation (SR) Fourier transform infrared (FTIR) imaging via a Focal plane array (FPA) detector and FTIR nanospectroscopy on fixed cells to study the molecular interactions of micro/nanoplastics within mammalian cells.Microplastic pollution has attracted attention in both the scientific community and general public. Defined as plastic particles and fibres <5 mm, these low-density materials are easily dispersed throughout the environment where they persist. As plastic remains widely used, the release of micro- and potentially nanoplastic (<100 nm) particles to the environment will continue as they degrade and shed. Whilst there is evidence for population exposure to microplastics via food, drinking water, dust and air, fundamentals about their toxicity remain unknown. (Vethaak 2021) Given the variety in which they occur, understanding how acute toxicity varies with composition (e.g., low/high density plastic materials), and size (micro/nano) are pertinent to predicting risk, informed by principals in particle toxicology.(Riediker 2019) Moreover, understanding whether microplastics biopersist inside cells and how these variables (polymer type, size) affect this is key to predicting the potential chronicity of their effects following exposure.

目的是开发一种结合活细胞同步辐射（SR）傅立叶变换红外（FTIR）成像（通过焦平面阵列（FPA）探测器）和固定细胞的FTIR纳米光谱的实验方法，以研究哺乳动物细胞内微/纳米塑料的分子相互作用。微塑料污染引起了科学界和公众的关注。这些低密度材料被定义为小于5 mm的塑料颗粒和纤维，它们很容易分散在环境中。随着塑料的广泛使用，微米和潜在的纳米塑料（<100 nm）颗粒在降解和脱落时将继续释放到环境中。虽然有证据表明人群通过食物，饮用水，灰尘和空气接触微塑料，但其毒性的基本原理仍然未知。（Vethaak 2021）考虑到它们发生的多样性，了解急性毒性如何随组成而变化（例如，低/高密度塑料材料）和尺寸（微米/纳米）与预测风险有关，由颗粒毒理学的负责人告知。（Riediker 2019）此外，了解微塑料是否在细胞内生物持久性以及这些变量（聚合物类型，大小）如何影响这一点是预测其暴露后影响的潜在慢性化的关键。