Nanoscale Molecular Characterisation of Hair Cuticles using Integrated AFM-IR
Nanoscale Molecular Characterisation of Hair Cuticles using Integrated AFM-IR
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使用集成 AFM-IR 对毛发角质层进行纳米级分子表征
DOI：
10.1101/2020.02.12.946103
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Fellows A
中科院分区：
文献类型：
--
作者：
Fellows A
利用振动光谱和成像技术在两个光谱区研究了毛发角质层的纳米级形貌和化学结构。原子力显微镜与可调谐红外激光(AFM-IR)的结合克服了传统红外光谱的衍射限制，促进了超空间分辨率的表面光谱。研究了靠近其边缘的角质层细胞表面蛋白质和脂肪含量的变化，以及边缘细胞本身裸露的层状结构。此外，还确定了胱氨酸相关产品对角质层的贡献。观察层中蛋白质、脂肪和胱氨酸组成的变化以及每一层的测量尺寸与角质层细胞亚结构的表皮层、A层、外角质层和内角质层的变化密切相关。到目前为止，大多数关于头发结构的化学研究工作仅限于整体测量，或者受到与传统红外光谱和显微镜相关的衍射限制。AFM-IR避开了这一衍射限制，允许进行纳米级、局部化学研究，并具有高表面选择性。虽然非化学研究，例如使用透射电子显微镜的研究，以前已经显示角质层具有分层的亚结构，但AFM-IR揭示了这些层中蛋白质和脂肪成分的显著化学变化，使其能够量化。
The nanometre-scale topography and chemical structure of hair cuticles has been investigated by vibrational spectroscopy and imaging in two spectral regions. The combination of Atomic Force Microscopy with a tuneable infrared laser (AFM-IR) circumvents the diffraction limit that has impaired traditional infrared spectroscopy, facilitating surface spectroscopy at ultra-spatial resolution. The variation in protein and lipid content of the cuticle cell surface approaching its edge, as well as the exposed layered structure of the cell at the edge itself, was investigated. Furthermore, the contribution of cystine-related products to the cuticle layers was determined. The variation of protein, lipid and cystine composition in the observed layers, as well as the measured dimensions of each, correspond closely to that of the epicuticle, A-layer, exocuticle and endocuticle layers of the cuticle cell sub-structure.Statement of SignificanceUsing AFM-IR to analyse the nanoscale cuticle features is both significant and novel in the field. Thus far, the great majority of work on the chemical investigation of the structure of hair has been limited to bulk measurements, or subject to the diffraction limit associated with traditional IR spectroscopies and microscopies. AFM-IR circumvents this diffraction limit and allows nanometre-scale, localised chemical investigation with high surface selectivity. While non-chemical investigations, e.g. those using Transmission Election Microscopy, have previously shown cuticles to have a layered substructure, AFM-IR sheds light on significant chemical variations of protein and lipid compositions within such layers, enabling their quantification.
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化学和光化学氧化导致人发角质层表面化学变化
影响因子：
2.3
作者：
S. Ruetsch;Y. Kamath
通讯作者：
Y. Kamath
DOI：
10.1071/bi9680375
发表时间：
1968-01-01
期刊：
AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES
影响因子：
--
作者：
KING, NLR;BRADBURY, JH
通讯作者：
BRADBURY, JH
影响因子：
1.7
作者：
Okamoto, Masayuki;Ishikawa, Kazutaka;Aoyagi, Satoka
通讯作者：
Aoyagi, Satoka
通过表面和内部改性防止头发脂质流失
影响因子：
4.6
作者：
Sang;J. H. Lim;S. Son;Julia Choi;N. Kang;Sang
通讯作者：
Sang
头发的形态和特性
DOI：
10.1201/9780203719565-1
发表时间：
2018
期刊：
Hair and Hair Care
影响因子：
--
作者：
M. Feughelman
通讯作者：
M. Feughelman