Core--Patient definition, 13C studies, postoperative tissue characterization

核心提供由两个或多个项目直接共享的资源或促进项目交互的资源。PI负责核心，并评估每个项目如何与其他项目交互。谷氨酸清除和能量代谢紊乱必须考虑到患者的选择、手术干预的进行、待研究组织的统一描述以及有关癫痫控制的结果。因此，核心部分将被分为四个部分：第一部分：术前患者群体的定义和推测的致痫底物。在本部分中，PI将首先定义将接受研究的患者，将获得知情同意，将电极放置在需要进行侵入性研究的患者身上，并将确保所有术前和定性、定量成像和电生理学已经进行(会议患者选择、MRI、体积测量、AVEEG、PET、MRS)。当患者接受侵入性研究时，将在体内和切片中提供谷氨酸的微透析评估。这些数据将在所有项目中得到相关使用。第13节：术中研究及组织分布。这包括术中对大脑进行数字化成像，以记录电生理异常的位置和海马体，并拍照、切片和编号，以供研究人员审查。第三部分：术后组织定征。这一部分致力于由Jung Kim博士进行的神经细胞和神经胶质细胞计数，为代谢和能量相关性研究提供细胞底物。第四节：项目协调。PI将在由一名行政助理协助的项目之间提供协调和数据共享，该助理将维持每周会议，协调所有术前测试中的患者移动，包括前往布鲁克海文国家实验室的旅行。管理员还负责收集和整理所有数据，协调跨学科研究，并安排内部和外部咨询会议。

The Core provides resources that are either directly shared by two or more projects or that promote project interaction. The PI is responsible for the Core and for assessing how each project interacts with the others. Glutamate clearance and energy metabolism derangement must be put into the context of patient selection, conduct of operative intervention, uniform description of tissue to be studied, and outcome regarding seizure control. The core will, therefore, be divided into four sections: Section 1: Preoperative definition of the patient population and presumed epileptogenic substrate. In this section, the PI will first define the patients that will undergo study, will obtain informed consent, will place electrodes in those patients requiring invasive study, and will make sure that all preoperative and qualitative and quantitative imaging and electrophysiology has been carried out (patient selection at conference, MRI, volumetrics, AVEEG, PET, MRS). Microdialysis assessment of glutamate will be provided both in vivo, when patients undergo invasive study, and in the slice. This data will be used correlatively in all the projects. Section: Intraoperative 13 study and tissue distribution. This involves digitized imaging of the brain intraoperatively to document the site of electrophysiological abnormalities and hippocampus in photographed, sectioned and numbered for investigator review. Section 3: Postoperative tissue characterization. This section is devoted to the neuronal and glial cell counts performed by Dr. Jung Kim which provides the cellular substrate for the metabolic and energy correlation studies. Section 4: Project coordination. The PI will provide coordination and data sharing among the projects assisted by an administrative associate who will maintain weekly conferences, coordinate patient movement among all the preoperative tests including the trips to Brookhaven National Laboratories. The administrator is also responsible for collecting and collating all data, coordinating interdisciplinary studies, and scheduling internal and external advisory meetings.