BORRELIA BURGDORFERI GENE EXPRESSION IN TICK MIDGUT--ROL

描述(取自申请表)： 这项研究应用的目的是研究大黄鱼的基因表达模式。 硬蜱载体中螺旋体疏螺旋体的研究及分子生物学意义 发生在螺旋体上的变化，以促进疾病传播。 为了实现这一目标，人们将利用一种新的方法来研究差异基因。 硬体适应螺旋体的表达。将进行试验以：a) 确定螺旋体在特定进食时间点表达的基因；b) 分析这些基因表达的时间和空间模式 致力于研究与生物相关的分子 疾病的传播；以及C)产生缺乏特异性的突变疏螺旋体 在这一应用中识别出感兴趣的基因并测试其潜力 这些突变的疏螺旋体传播疾病。基因的蛋白质产物 被推断在疾病传播中具有生理作用的基因将 最终被测试为传播阻断疫苗。这样做的结果 研究将使人们能够更好地了解参与 莱姆病的传播和允许新的干预策略 疾病的威胁。这项调查也将有助于制定一项 用于分析其他基因表达模式的有用的分子工具/方法 媒介传播的病原体在其媒介环境中。

DESCRIPTION (Taken from the application): The research application aims to study the gene expression pattern of the spirochete Borrelia in the Ixodes tick vector and to comprehend the molecular changes that occur on the spirochetes to facilitate disease transmission. Towards this goal, a novel approach will be utilized to study differential gene expression of tick-adapted spirochetes. Experiments will be carried out to: A) identify genes expressed by the spirochetes at specific feeding time points; B) analyze the temporal and spatial pattern of expression of these genes in an effort to focus on molecules that would be biologically relevant to transmission of disease; and C) to generate mutant Borrelia lacking specific genes of interest identified in this application and test the potential of these mutant Borrelia to transmit disease. The protein products of the genes that are inferred to have a physiological role in disease transmission will ultimately be tested as transmission blocking vaccines. The results of this investigation will enable a better understanding of molecules involved in transmission of Lyme disease and permit novel strategies for the intervention of disease. The investigation will also be conducive to the development of a useful molecular tool/approach to analyze the gene expression patterns of other vector-borne pathogens in their vector environment.