Regulation of Fungal Invasive Growth

描述（由申请人提供）：本研究项目调查了 调节真菌生物体的侵入性生长。在哺乳动物中， 侵袭正常组织屏障是转移性癌症重要特征 细胞和发育中的胚胎中的细胞。相反，在成年人中， 与基质的调节相互作用控制细胞增殖， 凋亡这种基质的调节作用在恶性肿瘤时通常会消失。 转型这项研究的目的是了解监管 与底层的相互作用。白色念珠菌和酵母菌 酿酒酵母将被用作模型系统，因为真菌细胞也相互作用 与substratum。在条件致病菌中，C.白色念珠菌，相互作用 基质在促进生物体的侵入性生长中可能是重要的 在宿主的组织中。因此，C.白色念珠菌侵袭性生长 也有助于理解一个过程， 疾病。 C.白色念珠菌通过产生侵入性的 丝状菌丝，其渗透到基质中。梭菌培养物白色 周围基质中的细胞促进菌丝的快速产生。遗传 对这一过程的分析导致了两种基因产物的鉴定 这是正常的菌丝生产所需要的，以响应周围的基质。 S.酿酒酵母也通过经历 侵入性生长，虽然，在这种生物体中，侵入与 形态上的巨大变化。S.酿酒酵母C.白色 上述基因是正常侵入性生长所需的。因此，研究 in S.酿酒酵母将进行，以开发详细的分子 对基因功能的假设。在C.白色念珠菌将测试 一般性的假设发展在S。啤酒。实验 提出鉴定与感兴趣的基因产物结合的相互作用物， 以阐明这些基因产物参与的途径。

DESCRIPTION (provided by applicant): This research project investigates the regulation of invasive growth in fungal organisms. In mammals, the ability to invade normal tissue barriers is an important attribute of metastatic cancer cells and of cells in the developing embryo. In contrast, in the adult regulatory interactions with the substratum control cell proliferation and apoptosis. This regulation by substratum is commonly lost upon malignant transformation. The goal of this research is to understand regulatory interactions with substratum. The fungi Candida albicans and Saccharomyces cerevisiae will be used as model systems because fungal cells also interact with substratum. In the opportunistic pathogen, C. albicans, interactions with the substratum may be important in promoting invasive growth of the organism within the tissues of a host. Thus, studies of C. albicans invasive growth will also contribute to the understanding of a process that plays an important role in disease. C. albicans responds to the presence of substratum by producing invasive filamentous hyphae, which penetrate into the matrix. Culture of C. albicans cells within surrounding matrix promotes rapid production of hyphae. Genetic analysis of this process has led to the identification of two gene products that are needed for normal hyphal production in response to surrounding matrix. S. cerevisiae also responds to the presence of substratum by undergoing invasive growth, although, in this organism, invasion is not associated with a dramatic change in morphology. S. cerevisiae homologues of the C. albicans genes described above are needed for normal invasive growth. Therefore, studies in S. cerevisiae will be performed in order to develop detailed molecular hypotheses for the function of the genes. Studies in C. albicans will test the generality of the hypotheses developed in S. cerevisiae. Experiments are proposed to identify interactors that bind to the gene products of interest and to elucidate the pathways in which these gene products participate.