CELLULAR DEFENSE RESPONSE IN AN INSECT SYSTEM

描述(根据申请者的申请改编)：这是一个5年制课程 竞争性续签题为“蜂窝防御反应”的提案 昆虫系统。在之前的提案中，PI开发了一个优雅的系统 幼虫血细胞包裹异物的实验研究 在飞蛾属中，假球蛾包括。这些研究具有重要意义，因为 包膜是昆虫免疫的基本组成部分。 回应。在这场竞争性的更新中，私家侦探将继续调查 参与这一过程的两种细胞类型，浆细胞和颗粒细胞 回应。他还将延长之前在浆细胞传播肽方面的工作 (PSP1)，它诱导浆细胞经历黏附状态的改变和 以释放特定的效应器分子。本申请具有4个特定 目的：1.鉴定PSP1活性必需的结构特征；2. 确定PSP1受体，3，以确定介导浆细胞的分子 粘附性，以及4，检测浆细胞诱导的特性 细胞毒性。太子参大量血细胞的可获得性 包括，以及在PI中开发的具体技术和方法 实验室都强烈支持这一实验系统，它 将提供对免疫功能的重要比较洞察 节肢动物媒介。

DESCRIPTION (Adapted from the Applicant's Application): This is a 5-year competitive renewal of a proposal entitled 'Cellular defense response in an insect system.' In the previous proposal the PI has developed an elegant system to investigate the encapsulation of a foreign object by hemocytes from larvae of the moth, Pseudoplusia includens. These studies are significant because encapsulation constitutes a fundamental component of the insect immune response. In this competing renewal, the PI will continue his investigation of the two cell types, plasmatocytes and granular cells that participate in this response. He will also extend previous work on a plasmatocyte spreading peptide (PSP1), which induces plasmatocytes to undergo a change in adhesive state and to release specific effector molecules. The present application has 4 specific aims: 1. To characterize structural features of PSP1 essential for activity; 2, to identify a PSP1 receptor, 3, to identify molecules that mediate plasmatocyte adhesion, and 4, to examine characteristics of plasmatocyte-induced cytotoxicity. The availability of large numbers of hemocytes from Pseudoplusia includens, and the specific techniques and approaches developed in the PI's laboratory are strong arguments in favor of this experimental system, which will provide important comparative insights into immunity functions in arthropod vectors.