BIOLOGY AND CONTROL OF LYME DISEASE BORRELIA

描述(改编自申请者摘要)：建议的协作 该项目是基于对发病机制和 莱姆病流行病学、生态学、媒介生物学和种群 媒介传播疾病的遗传学、疏螺旋体的分子生物学 伯格多菲病毒和疫苗的开发。建议的研究重点如下： 伯氏杆菌的进化生物学及莱姆病防治新策略 预防。 长期目标如下：(1)显著降低 通过疫苗干预在人和家畜中传播莱姆病 白纹伊蚊在高度流行区的自然维持周期。(2)至 通过控制研究伯氏杆菌作为感染源的演变 在高度流行地区进行干预研究。(3)提供一种疫苗 是非常有效的，而且可以快速、安全和廉价地 在野外使用。 该项目的假设如下：(A)高患病率 白斑Permyscus leucopus和(或)其他哺乳动物中的抗OspA抗体 油田中的水库会降低水库之间的传输率 宿主和媒介蚤，从而降低伯氏杆菌的流行率 扁虱的感染。(B)现场水塘的防疫注射不会改变 年疫苗现场伯氏杆菌种群结构 与控制点进行比较。(C)P。 白纹伊蚊和/或其他哺乳动物的蓄水池可以通过口腔和 用单一免疫原或联合免疫原进行非肠道免疫。 具体目标如下：(1)田间试验研究 白纹伊蚊免疫对伯氏杆菌传播率的影响 在水库宿主和媒介蚤之间。(2)影响评估 疫苗现场干预对白背飞虱种群结构的影响 在扁虱和脊椎动物的水库里。(3)进一步发展 单一疫苗接种和口服野战疫苗接种及效果评价 第二，补充抗原。 拟议的研究可能会提供分子、组织和 伯氏假单胞菌及其传播和宿主的种群水平 免疫应答。这一结果也可能与控制马立克氏病有关。 其他传播性人畜共患病和可能用于开发的新奇 提供疫苗的方法。

DESCRIPTION (Adapted from Applicant's Abstract): The proposed collaborative project is based collectively on investigations of the pathogenesis and epidemiology of Lyme disease, the ecology, vector biology, and population genetics of vector-borne diseases, the molecular biology of Borrelia burgdorferi, and vaccine development. The emphases of the proposed studies are the evolutionary biology of B. burgdorferi and new strategies for Lyme disease prevention. The long term goals are the following: (1) To significantly reduce the risk of Lyme disease among humans and domestic animals by vaccine intervention in the natural maintenance cycle of B. burgdorferi in a highly endemic area. (2) To study the evolution of B. burgdorferi as an infectious agent through controlled intervention studies in a highly endemic area. (3)To provide for a vaccine that is highly effective and that can be expeditiously, safely, and inexpensively administered in the field. The hypotheses for the project are the following: (A) A high prevalence of anti-OspA antibodies among Peromyscus leucopus and/or other mammalian reservoirs in the field will reduce the transmission rate between reservoir hosts and vector ticks, thereby reducing the prevalence of B. burgdorferi infection in ticks. (B) Immunization of reservoirs in the field will not alter the population structure of B. burgdorferi at the vaccine field sites in comparison to the control sites. (C) Transmission-blocking immunity among P. leucopus and/or other mammalian reservoirs can be achieved by oral as well as by parenteral immunization with a single immunogen or combined immunogens. The specific aims are the following: (1) Field studies of the effect of immunization of P. leucopus on the rate of transmission of B. burgdorferi between reservoir host and vector ticks. (2) Assessment of the effects of vaccine intervention in the field on the population structure of B. burgdorferi in ticks and in vertebrate reservoirs. (3) Further development of single-vaccination and orally-delivered field vaccines and evaluation of second, supplementary antigens. The proposed studies may provide insights as molecular, organismal, and population levels about B. burgdorferi, its transmission, and reservoir host immunize responses. The results may also have relevance for the control of other vector-bone zoonotic diseases and perhaps for development of novel methods for vaccine delivery.