Study of Cell disruption by ozonation for the production of biofuel

本研究的目的是在生物反应器中对二级处理废水中的微藻和酵母进行共培养。在微藻和酵母培养前后，通过测量pH值、浊度、COD、氨氮、硝酸盐、正磷酸盐、电导率和总悬浮物来表征废水，以监测废水处理过程的效率。为了获得生物质，将使用臭氧/臭氧浮选工艺，通过改变流速、臭氧氧化时间和臭氧浓度来优化工艺。本研究将对臭氧浮选技术破坏微藻细胞的效果进行实证研究。此外，本研究的目的是将臭氧漂浮与其他技术相结合，寻找最佳的收获方法。为了达到准确的结果，所有的实验都将进行多次。本研究旨在将分离微藻和收获脂肪结合在同一工艺中，以降低能源需求。此外，它的目的是通过臭氧氧化来量化微藻的细胞破裂。

The purpose of this research is to co-culture microalgae and yeast in secondary treated wastewater in a bioreactor. Wastewater will be characterized via measuring pH, turbidity, COD, ammonia, nitrate, orthophosphate, conductivity, and total suspended solids, before and after microalgae and yeast cultivation to monitor the efficiency of the wastewater treatment process. For harvesting biomass an ozonation/ozoflotation process will be used with the variation in the flow rate, ozonation time and ozone concentration to optimize the process. This research will empirically investigate the effect of cell disruption of microalgae using ozoflotation. Furthermore, the research aims to find the optimum harvesting approach by combining ozoflotation with other techniques. All the experiments will be done multiple times in order to achieve accurate results. This research aims to combine the separation of microalgae and harvesting of lipid in the same process to reduce the energy demand. Also, it aims to quantify the cell rupture of microalgae by ozonation.