Federally Registered Human Embryonic Stem Cell Center

描述（由申请人提供）：随着人类胚胎干（hES）细胞的衍生，以及随后对2001年8月9日之前衍生的细胞系的联邦资助资格的限制，合格细胞系的有效分配至关重要。这项建议旨在通过三个目标实现这一目标。目标1：首先，来自加州大学弗朗西斯科分校（UCSF）的hES细胞，HSF-6和HSF-1将大量扩增，测试无菌性，hES细胞特性和分化潜力;分布到世界各地的学术机构。还将衍生用于分布的hES系的亚系，所述亚系选择用于有用的特征，例如绿色荧光蛋白的表达和hES细胞表面标志物的高水平表达。一个网站将为用户提供有关细胞系的信息和订购表格。目标二：第二，将为合格的技术人员启动培训计划，为接受实验室提供在自己的实验室成功建立hES培养所需的专业知识。最后，UCSF可用的hES细胞系将逐渐转移到包含良好实验室规范的培养物中，包括细胞历史和培养条件的广泛测试和记录。这些目标的实现将为用户提供进行人类发育和人类移植组织生成的创新研究所需的宝贵工具。

DESCRIPTION (PROVIDED BY APPLICANT): With the dervation of human embryonic stem (hES) cells, and the subsequent limitation of eligibility for federal funding to those lines derived prior to August 9, 2001, the efficient distribution of the eligible lines is of primary importance. This proposal seeks to achieve this goal through three aims. Aim 1: First, The hES cells derived at University of California, San Francisco (UCSF), HSF-6 and HSF-1 will be expanded in large lots, tested for sterility, hES cell characteristics, and differentiation potential; for distribution to academic facilities throughout the world. Sublines of hES lines selected for useful characteristics, such as expression of green fluorescent protein and high levels of expression of hES cell-surface markers will also be derived for distribution. A website will provide users with information about the cell lines, and order forms. Aim 2: Secondly, a training program will be initiated for qualified technical staff to provide recipient labs with the expertise necessary to successfully establish hES cultures in their own lab. Finally, the hES cell lines available at UCSF will be gradually transferred to cultures incorporating good laboratory practices, including extensive testing and documentation of the history of the cells and culture conditions the lines have encountered. Achievement of these aims will provide users with the valuable tools necessary to perform innovative studies of human development and generation of tissues for human transplantation.