MECHANISM OF SPINDLE ASSEMBLY AND CHROMOSOME SEGREGATION

描述：目的1是进一步确定XKCM 1如何通过以下方式使微管不稳定： 一系列领域研究。第二个系列的研究将集中在 建立微管不稳定的进化保守性 其他Kini家族成员的活动。目标2是识别和表征 与XKCM1相互作用的蛋白质。在此，申请人特别 感兴趣的是识别潜在的动粒成分， XKCM1与染色体的关联。目的三是探讨 非洲爪蟾提取物中的染色体分离。目的4是使用试剂，开发 在以前的研究中，破坏XKCMP 1在体内的功能。目标5：评估 XKCMP 1功能已被操纵的细胞中的染色体运动，如 之前的目标。

DESCRIPTION: Aim 1 is to further define how XKCM1 destabilizes microtubules by a series of domain studies. A second series of studies will be focused on establishing evolutionary conservation of the microtubule destabilizing activity of other KinI family members. Aim 2 is to identify and characterize proteins that interact with XKCM1. Here the applicant is particularly interested in identifying potential kinetochore components involved in association of XKCM1 with chromosomes. Aim 3 is to investigate the mechanism of chromosome segregation in Xenopus extracts. Aim 4 is to use reagents, developed in previous studies, to disrupt XKCMP1 function in vivo. Aim 5 is to assess chromosome movement in cells where XKCMP1 function has been manipulated as in the previous aim.