Runx1 as a Marker for Stem Sells

描述（由申请人提供）：Runx1（AML 1）编码核心结合因子（CBF）的DNA结合亚基。Runx1是胚胎发生过程中造血干细胞（HSC）出现所必需的，并在HSC中表达。在这里，我们建议检查Runx1的表达和功能，在其他干细胞群体在成年小鼠骨髓和妊娠中期小鼠胎儿。几个实验室已经表明，从骨髓中分离的细胞可以促进骨骼肌、心肌和内皮细胞以及肝细胞的形成。我们将确定Runx1表达是否标志着骨髓中的干细胞能够为这些组织做出贡献，或者相反，Runx1表达是否可以用于从HSC中分离肌肉，心脏和肝脏干细胞。我们还将确定Runx1是否是骨骼肌、心肌和内皮细胞以及肝细胞干细胞发育出现所必需的。最后，我们计划确定Runx1在小鼠胚胎中新出现的HSC中表达的靶基因。

DESCRIPTION (provided by applicant): Runx1 (AML1) encodes a DNA-binding subunit of the core-binding factors (CBFs). Runx1 is required for the emergence of hematopoietic stem cells (HSCs) during embryogenesis, and is expressed in HSCs. Here we propose to examine Runx1 expression and function in other stem cell populations in the adult mouse bone marrow and midgestation mouse fetus. Several laboratories have shown that cells isolated from the bone marrow can contribute to the formation of skeletal muscle, heart muscle and endothelial cells, and liver hepatocytes. We will determine whether Runx1 expression marks stem cells in the bone marrow capable of contributing to these tissues, or conversely if Runxl expression can be used to separate muscle, heart, and liver stem cells from HSCs. We will also determine whether Runx1 is required for the developmental emergence of stem cells for skeletal muscle, heart muscle and endothelial cells, and liver hepatocytes. Finally, we plan to identify target genes for Runx1 that are expressed in newly emerging HSCs in the mouse embryo.