REGULATION OF THE MEIOTIC CELL CYCLE

描述：在有丝分裂周期中， 随后是染色体分离阶段，在此期间，复制的遗传 材料在子单元之间分配。减数分裂是一种特殊的 在一个细胞周期中，单个DNA复制阶段先于两个连续的 染色体分离期染色体减数分裂特异性调节因子 分离可能作用于有丝分裂和减数分裂的共同机制 染色体分离导致减数分裂染色体分离 程序.一种专门的泛素依赖的蛋白水解机制，APC （后期促进复合物），是染色体分离的关键调节因子 在有丝分裂期间。在酿酒酵母中，Pi已经表明APC 调节减数分裂重组，这对第一次减数分裂至关重要 染色体分离期Spo13蛋白是减数分裂特异性调节因子 染色体分离。 本提案的目标是：（1）确定咨询委员会在 减数分裂重组，（2）确定Spo13如何控制减数分裂染色体 分离和（3）鉴定新的基因，调节减数分裂染色体 种族隔离为了表征APC在减数分裂重组期间的作用， 将使用重组的遗传和分子测定来确定 在需要APC的重组期间的步骤。生化和遗传 然后，将使用各种方法来确定装甲运兵车目标， 进行减数分裂重组所必需的。两种方法将是 用于鉴定染色体分离的减数分裂特异性调节剂。第一、 已知Spo13调节减数分裂染色体分离， 研究以确定它如何调节这一过程并确定其目标。 第二，将采用遗传筛选来鉴定调节 减数分裂而非有丝分裂染色体分离。

DESCRIPTION: During the mitotic cycle a single round of DNA replication is followed by a chromosome segregation phase, during which the duplicated genetic material is partitioned between the daughter cells. Meiosis is a specialized cell cycle in which a single DNA replication phase precedes two consecutive chromosome segregation phases. Meiosis-specific regulators of chromosome segregation are likely to act on the machinery common to mitotic and meiotic chromosome segregation to bring about the meiotic chromosome segregation program. A specialized ubiquitin-dependent proteolysis machinery, the APC (anaphase promoting complex), is a key regulator of chromosome segregation during mitosis. In Saccharomyces cerevisiae, the Pi has shown that the APC regulates meiotic recombination, which is critical for the first meiotic chromosome segregation phase. The protein Spo13 is a meiosis-specific regulator of chromosome segregation. The goals of this proposal are (1) to determine the role of the APC during meiotic recombination, (2) to determine how Spo13 controls meiotic chromosome segregation and (3) to identify new genes that regulate meiotic chromosome segregation. To characterize the role of the APC during meiotic recombination, genetic and molecular assays for recombination will be used to determine the step during recombination at which the APC is required. Biochemical and genetic approaches will then be used to identify APC target(s) whose destruction is required for the execution of meiotic recombination. Two approaches will be taken to identify meiosis-specific regulators of chromosome segregation. First, Spo13, which is known to regulate meiotic chromosome segregation will be studied to determine how it regulates this process and to identify its targets. Second, a genetic screen will be employed to identify new genes that regulate meiotic but not mitotic chromosome segregation.