CORE B: Immunohistochemical Imaging, Flow Cytometry, and Virus Preparation

描述(由申请人提供) 免疫组织化学成像、流式细胞术和病毒制备核心旨在提供与确定中枢神经系统内细胞的表型和分布相关的技术专业知识和服务，以及为计划项目赠款(PPG)的成员提供病毒储备。PPG的总体目标是阐明泰勒氏病毒诱导的脱髓鞘疾病的诱导和进展的细胞和分子机制。一个关键的特征是能够确定中枢神经系统浸润性细胞的表型以及它们在疾病发展和进展过程中的空间分布。此外，对疾病调控的研究需要了解各种治疗方法如何影响正常的细胞迁移和积累模式。这一核心将执行PPG作为一个凝聚力单位的三个重要功能：目标1-中枢和周围淋巴组织的免疫组织化学分析和成像。目的2--流式细胞仪的监督和管理。

Description (provided by applicant) The Immunohistochemical Imaging, Flow Cytometry, and Virus Preparation Core is designed to provide technical expertise and services relating to determining the phenotype and distribution of cells within the central nervous system as well as to provide virus stocks for members of the Program Project Grant (PPG). The overall goal of the PPG is to elucidate the cellular and molecular mechanisms of induction and progression of Theiler's virus-induced demyelinating disease. A critical feature is the ability to determine the phenotype of CNS-infiltrating cells as well as their spatial distribution during the development and progression of disease. Furthermore, investigations into disease regulation requires knowledge of how various treatments affect the normal cellular migration and accumulation patterns. This Core will perform three vital functions for the execution of the PPG as a cohesive unit: Aim 1 - immunohistochemical analysis and imaging of CNS and peripheral lymphoid tissues. Aim 2 - Oversight and management of the flow cytometry instrumentation.