Elucidation and application of lignin degradation pathways in bacteria

木质素降解的代谢工程中的当前瓶颈是对氧化的木质素片段转化为单环芳族化合物（例如原儿茶酸或儿茶酚）的代谢途径的理解不足，然后通过已知的芳族化合物降解途径对单环芳族化合物进行加工。本项目的目的是阐明这些途径，使用报告分析和基因文库的筛选，并将这些途径应用于木质素降解的代谢工程，以产生新的生物产品。

A current bottleneck in metabolic engineering of lignin degradation is a poor understanding of the metabolic pathways by which oxidised lignin fragments are converted into monocyclic aromatics such as protocatechuic acid or catechol, which are then processed by known aromatic degradation pathways. The aim of this project is to elucidate these pathways, using reporter assays and screening of gene libraries, and to apply these pathways for metabolic engineering of lignin degradation to generate new bioproducts.